Hemalatha M, Subathra Devi C
Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
Front Microbiol. 2022 Aug 25;13:982260. doi: 10.3389/fmicb.2022.982260. eCollection 2022.
In the present study, -HDS27 strain isolated from bovine milk was used for the enhanced production of riboflavin. Production medium was optimized by one factor at a time with different parameters. Statistical optimization by Response surface methodology (RSM), central composite design was used to optimize variables such as pH, temperature, glucose, and yeast extract. The present study reveals the maximum riboflavin production by one factor at a time was obtained under the culture conditions; glucose, yeast extract, pH 6, the temperature at 40°C, and 3% of inoculum size. In RSM, analysis of variance for the responses was calculated. Among the tested variables, pH, yeast extract, and temperature showed significant impact on riboflavin production. Maximum amount of yeast extract in production medium resulted in increased riboflavin production. The riboflavin production after 24 h with the optimal condition was found to be 12.33 mg/L. It was found proximate to the expected value (12.29 mg/L) achieved by the RSM model. The yield of riboflavin was increased to 3.66-fold after 24 h with the optimized parameters. The current research, emphasizes that the -HDS27 could be an excellent strain for the large-scale industrial production of riboflavin.
在本研究中，从牛乳中分离出的-HDS27菌株用于提高核黄素的产量。通过一次改变一个参数对生产培养基进行优化。采用响应面法（RSM）进行统计优化，运用中心复合设计对pH、温度、葡萄糖和酵母提取物等变量进行优化。本研究表明，一次改变一个因素时，在葡萄糖、酵母提取物、pH 6、温度40°C和接种量3%的培养条件下可获得最大核黄素产量。在RSM中，计算了响应的方差分析。在所测试的变量中，pH、酵母提取物和温度对核黄素产量有显著影响。生产培养基中酵母提取物的最大量导致核黄素产量增加。在最佳条件下培养24小时后，核黄素产量为12.33 mg/L。发现其接近RSM模型获得的预期值（12.29 mg/L）。优化参数后，24小时内核黄素产量提高到3.66倍。当前研究强调，-HDS27可能是大规模工业生产核黄素的优良菌株。
