Foiani Greta, Mandara Maria Teresa, Carminato Antonio, Melchiotti Erica, Corrò Michela, Vascellari Marta
Laboratory of Histopathology, Istituto Zooprofilattico Sperimentale delle Venezie, SCS3, Legnaro, Italy.
Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Front Vet Sci. 2022 Aug 25;9:961056. doi: 10.3389/fvets.2022.961056. eCollection 2022.
An 8-month-old female Main Coon with a history of recurrent behavioral changes and anorexia was presented with sternal recumbency and depression. Within 5 days, the cat progressively worsened with symptoms of stupor and coma and was euthanized. At post-mortem examination, a solid, grayish infratentorial mass located in the midline rostrally to the cerebellum, was observed. Histologically, highly cellular clusters of small-to-medium undifferentiated cells were intermingled with paucicellular areas with fibrillary eosinophilic (neuropil-like) appearance. Numerous multilayered (ependymoblastic) true rosettes were present. The mitotic activity was frequent (up to 15 mitoses/HPF), involving both undifferentiated cells and rosettes. By immunohistochemistry (IHC), tumor cells were diffusely positive for vimentin, variably for synaptophysin, S-100, and NSE, and focally for NeuN; they were negative for GFAP and CK AE1/AE3. The histological and IHC aspects were consistent with an Embryonal Tumor with Abundant Neuropil and True Rosettes (ETANTR). Embryonal neoplasms of the central nervous system (CNS) are characterized by primitive undifferentiated cells, able to develop toward neuronal, glial, ependymal, and mesenchymal lines. Although extremely rare, juvenile embryonal tumors should be considered in the differentials of CNS disorders in young cats.
一只8个月大的雌性缅因猫，有反复行为改变和厌食病史，出现胸骨卧位和精神沉郁。在5天内，这只猫病情逐渐恶化，出现木僵和昏迷症状，最终实施了安乐死。尸检时，在小脑前方中线处发现一个位于幕下的实性、灰白色肿块。组织学上，中小未分化细胞的高细胞簇与具有纤维状嗜酸性（神经毡样）外观的少细胞区域相互交织。存在许多多层（室管膜母细胞样）真性菊形团。有丝分裂活动频繁（高达15个有丝分裂/高倍视野），累及未分化细胞和菊形团。通过免疫组织化学（IHC），肿瘤细胞波形蛋白弥漫性阳性，突触素、S-100和神经元特异性烯醇化酶呈不同程度阳性，神经元核抗原局灶性阳性；它们胶质纤维酸性蛋白和细胞角蛋白AE1/AE3阴性。组织学和免疫组织化学表现与富含神经毡和真性菊形团的胚胎性肿瘤（ETANTR）一致。中枢神经系统（CNS）的胚胎性肿瘤以原始未分化细胞为特征，能够向神经元、胶质、室管膜和间充质谱系发展。尽管极为罕见，但在幼猫中枢神经系统疾病的鉴别诊断中应考虑幼年胚胎性肿瘤。
