Belij-Rammerstorfer Sandra, Limon Georgina, Maze Emmanuel A, Hannant Kayleigh, Hughes Ellen, Tchakarova Simona R, Alexandrov Tsviatko, Mmbaga Blandina T, Willett Brian, Booth George, Lyons Nicholas A, Baker Natalie, Thomas Kelly M, Wright Daniel, Saunders Jack, Browning Clare, Wilsden Ginette, Carroll Miles, Hewson Roger, Charleston Bryan, Lambe Teresa, Ludi Anna B
Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, United Kingdom.
The Pirbright Institute, Woking, United Kingdom.
Front Vet Sci. 2022 Aug 25;9:913046. doi: 10.3389/fvets.2022.913046. eCollection 2022.
Crimean-Congo hemorrhagic fever (CCHF) is a priority emerging disease. CCHF, caused by the CCHF virus (CCHFV), can lead to hemorrhagic fever in humans with severe cases often having fatal outcomes. CCHFV is maintained within a tick-vertebrate-tick cycle, which includes domestic animals. Domestic animals infected with CCHFV do not show clinical signs of the disease and the presence of antibodies in the serum can provide evidence of their exposure to the virus. Current serological tests are specific to either one CCHFV antigen or the whole virus antigen. Here, we present the development of two in-house ELISAs for the detection of serum IgG that is specific for two different CCHFV antigens: glycoprotein Gc (CCHFV Gc) and nucleoprotein (CCHFV NP). We demonstrate that these two assays were able to detect anti-CCHFV Gc-specific and anti-CCHFV NP-specific IgG in sheep from endemic CCHFV areas with high specificity, providing new insight into the heterogeneity of the immune response induced by natural infection with CCHFV in domestic animals.
克里米亚-刚果出血热（CCHF）是一种重点关注的新发疾病。由克里米亚-刚果出血热病毒（CCHFV）引起的CCHF可导致人类出现出血热，严重病例往往会导致死亡。CCHFV在蜱-脊椎动物-蜱的循环中传播，其中包括家畜。感染CCHFV的家畜不会表现出该疾病的临床症状，血清中抗体的存在可证明它们接触过该病毒。目前的血清学检测针对的要么是一种CCHFV抗原，要么是整个病毒抗原。在此，我们展示了两种用于检测血清IgG的内部酶联免疫吸附测定（ELISA）的开发，这些IgG对两种不同的CCHFV抗原具有特异性：糖蛋白Gc（CCHFV Gc）和核蛋白（CCHFV NP）。我们证明，这两种检测方法能够以高特异性检测来自CCHFV流行地区绵羊体内的抗CCHFV Gc特异性IgG和抗CCHFV NP特异性IgG，为家畜自然感染CCHFV诱导的免疫反应的异质性提供了新的见解。
