Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Suedufer 10, 17489 Greifswald-Insel Riems, Germany.
Department of Medicine, Karolinska Institutet, SE-17177 Stockholm, Sweden.
Viruses. 2021 Feb 26;13(3):372. doi: 10.3390/v13030372.
Dugbe orthonairovirus (DUGV) is a tick-borne arbovirus within the order . DUGV was first isolated in Nigeria, but virus isolations in ten further African countries indicate that DUGV is widespread throughout Africa. Humans can suffer from a mild febrile illness, hence, DUGV is classified as a biosafety level (BSL) 3 agent. In contrast, no disease has been described in animals, albeit serological evidence exists that ruminants are common hosts and may play an important role in the transmission cycle of this neglected arbovirus. In this study, young sheep and calves were experimentally inoculated with DUGV in order to determine their susceptibility and to study the course of infection. Moreover, potential antibody cross-reactivities in currently available diagnostic assays for Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) were assessed as DUGV is distantly related to CCHFV. Following subcutaneous inoculation, none of the animals developed clinical signs or viremia. However, all ruminants seroconverted, as demonstrated by two DUGV neutralization test formats (micro-virus neutralization test (mVNT), plaque reduction (PRNT)), by indirect immunofluorescence assays and in bovines by a newly developed DUGV recombinant N protein ELISA. Sera did not react in commercial CCHFV ELISAs, whereas cross-reactivities were observed by immunofluorescence and immunoblot assays.
杜格贝伊正呼肠孤病毒（DUGV）是一种属于呼肠孤病毒目的蜱传病毒。DUGV 最初在尼日利亚被分离出来，但在另外十个非洲国家的病毒分离表明，DUGV 在整个非洲广泛传播。人类可能会患上轻度发热性疾病，因此，DUGV 被归类为生物安全级别（BSL）3 制剂。相比之下，在动物中没有描述疾病，尽管有血清学证据表明反刍动物是常见的宿主，并且可能在这种被忽视的虫媒病毒的传播循环中发挥重要作用。在这项研究中，年轻的绵羊和小牛被实验性地接种了 DUGV，以确定它们的易感性并研究感染过程。此外，还评估了目前用于克里米亚-刚果出血热正呼肠孤病毒（CCHFV）的诊断检测中潜在的抗体交叉反应性，因为 DUGV 与 CCHFV 有较远的亲缘关系。皮下接种后，没有动物出现临床症状或病毒血症。然而，所有反刍动物都发生了血清转化，如两种 DUGV 中和试验（微病毒中和试验（mVNT）、蚀斑减少（PRNT））、间接免疫荧光测定以及在牛中通过新开发的 DUGV 重组 N 蛋白 ELISA 所证明。血清在商业 CCHFV ELISA 中没有反应，而在免疫荧光和免疫印迹测定中观察到交叉反应性。
