Chen Linhai, Yang Jing, Kim Sang Ah, Gelvosa Ma Nessa, Wei Peng, Jeon Jae Yong, Cheon Hwayeong
Department of Plastic and Reconstructive Surgery, Ningbo First Hospital, Ningbo, China.
Department of Anesthesiology, The First Affiliated Hospital of Zhejiang University Medical College, Hangzhou, China.
Front Vet Sci. 2022 Aug 25;9:912278. doi: 10.3389/fvets.2022.912278. eCollection 2022.
Constructing a reliable animal model for preclinical treatment of secondary lymphedema is challenging because the anatomical characteristics near the lymph nodes are understudied. Therefore, this study examined the detailed anatomical relationship between the axillary lymph node flaps (ALNFs) and brachial lymph node flaps (BLNFs) in the forelimb of Sprague-Dawley (SD) rats.
Ten male rats, weighing 250-300 g, were used. The ALNFs and BLNFs on either side of the rat forelimbs were dissected. The two lymph node flaps (LNFs) were immediately harvested to analyze their physical characteristics (via imaging process software) and microscopic structure (via histology examinations).
A total of 20 ALNFs and BLNFs from 10 rats were harvested and analyzed. ALNF dissection was simpler and lasted a shorter time than BLNF dissection ( < 0.0001). The left LNFs were more difficult to dissect than the right LNFs ( < 0.0001). In physical characteristics of LNFs, the area ( < 0.001) of LNFs and the number of lymph nodes ( < 0.0001) associated with ALNFs were greater than those associated with BLNFs, but the pedicle lengths of ALNFs were shorter than that of BLNFs ( < 0.0001). No significant difference in the diameter of the venous and arterial pedicles was noted between the two LNFs ( > 0.05).
This study reported detailed physical characteristics of ALNFs and BLNFs in SD rat forelimbs, assessing the respective area of LNFs, number of lymph nodes, and lengths and diameters of vascular pedicles. Moreover, this study suggested an efficient method to perform a study of LNFs by describing the operation process and repeatedly measuring the operation time.
构建用于继发性淋巴水肿临床前治疗的可靠动物模型具有挑战性，因为淋巴结附近的解剖特征研究不足。因此，本研究考察了Sprague-Dawley（SD）大鼠前肢腋窝淋巴结皮瓣（ALNFs）与臂淋巴结皮瓣（BLNFs）之间的详细解剖关系。
使用10只体重250 - 300克的雄性大鼠。解剖大鼠前肢两侧的ALNFs和BLNFs。立即收获两个淋巴结皮瓣（LNFs）以分析其物理特征（通过成像处理软件）和微观结构（通过组织学检查）。
共收获并分析了来自10只大鼠的20个ALNFs和BLNFs。ALNF解剖比BLNF解剖更简单且耗时更短（<0.0001）。左侧LNFs比右侧LNFs更难解剖（<0.0001）。在LNFs的物理特征方面，LNFs的面积（<0.001）和与ALNFs相关的淋巴结数量（<0.0001）大于与BLNFs相关的，但ALNFs的蒂长度比BLNFs的短（<0.0001）。两个LNFs之间在静脉蒂和动脉蒂直径上未观察到显著差异（>0.05）。
本研究报告了SD大鼠前肢ALNFs和BLNFs的详细物理特征，评估了LNFs的各自面积、淋巴结数量以及血管蒂的长度和直径。此外，本研究通过描述操作过程并反复测量操作时间，提出了一种进行LNFs研究的有效方法。
