Ni Shuangfei, Luo Zixiang, Fan Yonggang, Zhang Weixin, Peng Wei, Zhang Huafeng
Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha, China.
Front Neurosci. 2022 Aug 25;16:904573. doi: 10.3389/fnins.2022.904573. eCollection 2022.
The mA methylation is reported to function in multiple physiological and pathological processes. However, the functional relevance of mA modification to post-spinal cord injured (SCI) damage is not yet clear. In the present study, methylated RNA immunoprecipitation combined with microarray analysis showed that the global RNA mA levels were decreased following SCI. Then, gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analyses were conducted to demonstrate the potential function of differential mA-tagged transcripts and the altered transcripts with differential mA levels. In addition, we found that the mA "writer," METTL3, significantly decreased after SCI in mice. The immunostaining validated that the expression of METTL3 mainly changed in GFAP or Iba-1 cells. Together, this study shows the alteration of mA modification following SCI in mice, which might contribute to the pathophysiology of the spinal cord after trauma.
据报道，N6-甲基腺嘌呤（mA）甲基化在多种生理和病理过程中发挥作用。然而，mA修饰与脊髓损伤（SCI）后损伤的功能相关性尚不清楚。在本研究中，甲基化RNA免疫沉淀结合微阵列分析表明，SCI后整体RNA mA水平降低。然后，进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析，以证明差异mA标记转录本和具有差异mA水平的改变转录本的潜在功能。此外，我们发现mA“书写者”METTL3在小鼠SCI后显著降低。免疫染色证实METTL3的表达主要在GFAP或Iba-1细胞中发生变化。总之，本研究显示了小鼠SCI后mA修饰的改变，这可能有助于创伤后脊髓的病理生理学。
