Reno Ulises, Regaldo Luciana, Ojeda Guillermo, Schmuck Josefina, Romero Natalí, Polla Wanda, Kergaravat Silvina V, Gagneten Ana María
Ecotoxicology Laboratory, Department of Natural Sciences, Faculty of Humanities and Sciences, National University of Littoral (UNL), 3000 Santa Fe, Argentina.
National Council for Scientific and Technological Research (CONICET), 3000 Santa Fe, Argentina.
Water Air Soil Pollut. 2022;233(9):372. doi: 10.1007/s11270-022-05772-w. Epub 2022 Sep 3.
The COVID-19 pandemic affected human life at every level. In this study, we analyzed genetic markers (N and ORF1ab, RNA genes) of SARS-CoV-2 in domestic wastewaters (DWW) in San Justo City (Santa Fe, Argentina), using reverse transcription-quantitative real-time PCR. Out of the 30 analyzed samples, 30% were positive for SARS-CoV-2 RNA. Of the total positive samples, 77% correspond to untreated DWW, 23% to pre-chlorination, and no SARS-CoV-2 RNA was registered at the post-chlorination sampling site. The viral loads of N and OFR1ab genes decreased significantly along the treatment process, and the increase in the number of viral copies of the N gene could anticipate, by 6 days, the number of clinical cases in the population. The concentration of chlorine recommended by the WHO (≥ 0.5 mg L after at least 30 min of contact time at pH 8.0) successfully removed SARS-CoV-2 RNA from DWW. The efficiency of wastewater-based epidemiology (WBE) confirms the need to control and increase DWW treatment systems on a regional and global scale. This work could contribute to building a network for WBE to monitor SARS-CoV-2 in wastewaters during the pandemic waves and the epidemic remission phase.
The online version contains supplementary material available at 10.1007/s11270-022-05772-w.
新冠疫情在各个层面影响着人类生活。在本研究中，我们使用逆转录定量实时聚合酶链反应，分析了阿根廷圣达菲省圣胡斯托市生活污水中严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的基因标记（N和ORF1ab，RNA基因）。在分析的30个样本中，30%的样本SARS-CoV-2 RNA呈阳性。在所有阳性样本中，77%对应未经处理的生活污水，23%对应预氯化处理的污水，而在氯化处理后的采样点未检测到SARS-CoV-2 RNA。在处理过程中，N基因和OFR1ab基因的病毒载量显著下降，N基因病毒拷贝数的增加可提前6天预测人群中的临床病例数。世界卫生组织推荐的氯浓度（在pH值为8.0时接触至少30分钟后≥0.5 mg/L）成功地从生活污水中去除了SARS-CoV-2 RNA。基于废水的流行病学（WBE）的有效性证实了在区域和全球范围内控制和增加生活污水处理系统的必要性。这项工作有助于建立一个WBE网络，以在疫情高峰期和疫情缓解阶段监测污水中的SARS-CoV-2。
在线版本包含可在10.1007/s11270-022-05772-w获取的补充材料。
