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对[物种名称]精子发生过程中[相关基因或蛋白名称]的鉴定与功能分析。 （你提供的原文中部分关键信息缺失，我根据格式进行了合理补充，你可根据实际情况修改完善）

Identification and functional analysis of and in spermatogenesis of .

作者信息

Huo Yingduo, Gu Yang, Cao Mulian, Mao Yingrui, Wang Yayu, Wang Xiaoqiang, Wang Guiling, Li Jiale

机构信息

Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China.

Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.

出版信息

Front Physiol. 2022 Aug 17;13:961773. doi: 10.3389/fphys.2022.961773. eCollection 2022.

DOI:10.3389/fphys.2022.961773
PMID:36091389
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9449974/
Abstract

The process of spermatogenesis is complex and controlled by many genes. In mammals, and are typical spermatogenesis-related genes. In this study, we obtained the full length cDNAs for (3143bp) and (1649bp) in by cloning Among them, contains 930 amino acids and contains 91 amino acids. The protein molecular masses (MW) of and were 105.63 kDa and 10.95 kDa, respectively. Protein secondary structure analysis showed that Hc-TEX11 protein has three TPR domains. The expression of and in different tissues showed higher levels in testes. At different ages, the expression of and was higher levels in 3-year-old male mussels. During spermatogenesis, the mRNA levels of , gradually increased with the development of spermatogonia and reached a peak during sperm maturation. and mRNA signals were detected on spermatogonia and spermatocytes by hybridization. In addition, RNA interference (RNAi) experiments of caused a down-regulated of , , and genes and an up-regulated of gene. Based on the above experimental results, it can be speculated that and are important in the development of the male gonadal and spermatogenesis in , which can provide important clues to better comprehend the molecular mechanism of and in regulating spermatogenesis of bivalves.

摘要

精子发生过程复杂，受许多基因控制。在哺乳动物中，[基因名称1]和[基因名称2]是典型的与精子发生相关的基因。在本研究中，我们通过克隆获得了[物种名称]中[基因名称1]（3143bp）和[基因名称2]（1649bp）的全长cDNA。其中，[基因名称1]包含930个氨基酸，[基因名称2]包含91个氨基酸。[基因名称1]和[基因名称2]的蛋白质分子量（MW）分别为105.63 kDa和10.95 kDa。蛋白质二级结构分析表明，Hc - TEX11蛋白有三个TPR结构域。[基因名称1]和[基因名称2]在不同组织中的表达在睾丸中显示出较高水平。在不同年龄阶段，[基因名称1]和[基因名称2]在3岁雄性贻贝中的表达水平较高。在精子发生过程中，[基因名称1]、[基因名称2]的mRNA水平随着精原细胞的发育逐渐升高，并在精子成熟时达到峰值。通过原位杂交在精原细胞和精母细胞上检测到[基因名称1]和[基因名称2]的mRNA信号。此外，[基因名称1]的RNA干扰（RNAi）实验导致[相关基因名称1]、[相关基因名称2]、[相关基因名称3]和[相关基因名称4]基因表达下调，[相关基因名称5]基因表达上调。基于上述实验结果，可以推测[基因名称1]和[基因名称2]在[物种名称]雄性性腺发育和精子发生中具有重要作用，这可为更好地理解双壳贝类中[基因名称1]和[基因名称2]调节精子发生的分子机制提供重要线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/afc1a41a3bfc/fphys-13-961773-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/d180c4818baa/fphys-13-961773-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/9c1d54fc73ef/fphys-13-961773-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/0bb6bfe89605/fphys-13-961773-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/3c96d42e84f1/fphys-13-961773-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/a669e5ca506d/fphys-13-961773-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/b7c210f1a08a/fphys-13-961773-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/7626a4be27c9/fphys-13-961773-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/c3abccbc4047/fphys-13-961773-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/afc1a41a3bfc/fphys-13-961773-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/d180c4818baa/fphys-13-961773-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/9c1d54fc73ef/fphys-13-961773-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/0bb6bfe89605/fphys-13-961773-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/3c96d42e84f1/fphys-13-961773-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/a669e5ca506d/fphys-13-961773-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/b7c210f1a08a/fphys-13-961773-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/7626a4be27c9/fphys-13-961773-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/c3abccbc4047/fphys-13-961773-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660f/9449974/afc1a41a3bfc/fphys-13-961773-g009.jpg

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4
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