Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai, 201306, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai, 201306, China.
Sci Rep. 2021 Jan 12;11(1):665. doi: 10.1038/s41598-020-80264-7.
Hyriopsis cumingii is an important species for freshwater pearl cultivation in China. In terms of pearl production, males have larger pearls and better glossiness than females, but there are few reports focusing on the sex of H. cumingii. In this study, six mRNA and six microRNA (miRNA) libraries were prepared from ovaries and testes. Additionally, 28,502 differentially expressed genes (DEGs) and 32 differentially expressed miRNAs (DEMs) were identified. Compared with testis, 14,360 mRNAs and 20 miRNAs were up-regulated in ovary, 14,142 mRNAs and 12 miRNAs were down-regulated. In DEGs, the known genes related to sex determinism and/or differentiation were also identified, such as DMRT1, SOX9, SF1 for males, FOXL2 for females, and other potentially significant candidate genes. Three sex-related pathways have also been identified, which are Wnt, Notch, and TGF-beta. In 32 DEMs, the three miRNAs (miR-9-5p, miR-92, miR-184) were paid more attention, they predicted 28 target genes, which may also be candidates for sex-related miRNAs and genes. Differential miRNAs target genes analysis reveals the pathway associated with oocyte meiosis and spermatogenesis. Overall, the findings of the study provide significant insights to enhance our understanding of sex differentiation and/or sex determination mechanisms for H. cumingii.
三角帆蚌是中国淡水珍珠养殖的重要物种。就珍珠生产而言，雄性的珍珠比雌性更大、更有光泽，但关于三角帆蚌性别的报道很少。在这项研究中，我们从卵巢和睾丸中制备了六个 mRNA 和六个 microRNA（miRNA）文库。此外，鉴定出了 28502 个差异表达基因（DEGs）和 32 个差异表达 microRNA（DEMs）。与睾丸相比，卵巢中上调的 mRNAs 和 miRNAs 分别有 14360 个和 20 个，下调的 mRNAs 和 miRNAs 分别有 14142 个和 12 个。在 DEGs 中，还鉴定出了与性别决定和/或分化相关的已知基因，如雄性的 DMRT1、SOX9、SF1，雌性的 FOXL2，以及其他潜在的重要候选基因。还鉴定出了三个与性别相关的途径，即 Wnt、Notch 和 TGF-β。在 32 个 DEMs 中，三个 miRNA（miR-9-5p、miR-92、miR-184）受到了更多关注，它们预测了 28 个靶基因，这些基因也可能是与性别相关的 miRNA 和基因的候选基因。差异 miRNA 靶基因分析揭示了与卵母细胞减数分裂和精子发生相关的途径。总的来说，这项研究的结果为增强我们对三角帆蚌性别分化和/或性别决定机制的理解提供了重要的见解。
