The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, 230031 Anhui, China.
Anhui University of Chinese Medicine, Hefei, 230012 Anhui, China.
Mediators Inflamm. 2022 Aug 31;2022:6561048. doi: 10.1155/2022/6561048. eCollection 2022.
Bronchial asthma (BA) is a chronic inflammatory disease of the airway. Previous research has shown that Yanghe Pingchuan granules (YPGs) exert a precise therapeutic effect on BA. In our previous work, we showed that YPGs improved inflammation of the airways in rat models of BA. Other studies have shown that the pathogenesis of BA is closely related to pyroptosis and that the TOLL-like receptor pathway plays a key role in the mediation of pyroptosis. Therefore, in the present study, we established a rat model of BA by applying the concept of pyroptosis and used the TLR4/NF-B/NRLP3 signaling pathway as the target and YPGs as the treatment method. We evaluated the effects of YPGs on airway inflammation and pyroptosis in the model rats by HE staining, Masson's staining, AP-PAS staining, western blotting, and real-time quantitative PCR. The results showed that Yanghe Pingchuan granules could significantly improve the inflammatory response of bronchial tissue in BA rats, reduce the content of inflammatory factors IL-1 and IL-18, and inhibit the expression of pyroptosis factor. Meanwhile, YPG can block the TLR4/NF-B signaling pathway. These findings suggest that YPG may be an effective drug for the treatment of BA by blocking the TLR4/NF-B signaling pathway and inhibiting pyroptosis.
支气管哮喘（BA）是一种气道慢性炎症性疾病。既往研究表明， 哮喘平喘颗粒（YPGs）对 BA 具有确切的治疗作用。在我们之前的工作中，我们表明 YPGs 改善了 BA 大鼠模型中的气道炎症。其他研究表明，BA 的发病机制与细胞焦亡密切相关，而 Toll 样受体（TLR）途径在介导细胞焦亡中起着关键作用。因此，本研究采用细胞焦亡的概念建立 BA 大鼠模型，以 TLR4/NF-B/NRLP3 信号通路为靶点，以 YPGs 为治疗方法，评价 YPGs 对模型大鼠气道炎症和细胞焦亡的影响。通过 HE 染色、Masson 染色、AP-PAS 染色、Western blot 和实时定量 PCR 评估 YPGs 对 BA 大鼠支气管组织炎症和细胞焦亡的影响。结果表明，哮喘平喘颗粒能显著改善 BA 大鼠支气管组织的炎症反应，降低炎症因子 IL-1 和 IL-18 的含量，抑制细胞焦亡因子的表达。同时，YPG 能阻断 TLR4/NF-B 信号通路。这些发现提示 YPG 可能通过阻断 TLR4/NF-B 信号通路抑制细胞焦亡而成为治疗 BA 的有效药物。
