Dadyar Maryam, Hussen Bashdar Mahmud, Eslami Solat, Taheri Mohammad, Emadi Farhad, Ghafouri-Fard Soudeh, Sayad Arezou
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Kurdistan Region, Erbil, Iraq.
Front Genet. 2022 Aug 26;13:967157. doi: 10.3389/fgene.2022.967157. eCollection 2022.
Long non-coding RNAs (lncRNAs) have been demonstrated to in the pathophysiology of multiple sclerosis (MS). In order to appraise the role of T cell-related lncRNAs in this disorder, we assessed expressions of NEST, RMRP, TH2-LCR, MAFTRR and FLICR in MS patients and healthy individuals. We detected significant difference in the expression of RMRP and FLICR between cases and controls. There were substantial correlations between expressions of NEST, RMRP, TH2-LCR, MAFTRR and FLICR lncRNAs among patients, but not controls. The strongest correlations were found between RMRP and TH2-LCR, and between MAFTRR and RMRP with correlation coefficients of 0.69 and 0.59, respectively. ROC curve analysis revealed appropriate power of FLICR in differentiating between MS patients and healthy controls (AUC value = 0.84). Expression of NEST lncRNA was positively correlated with disease duration in MS patients, but negatively correlated with age at onset. In brief, we reported dysregulation of two T cell-related lncRNAs in MS patients and proposed FLICR as a putative marker for this disorder.
长链非编码RNA（lncRNAs）已被证明在多发性硬化症（MS）的病理生理学中发挥作用。为了评估与T细胞相关的lncRNAs在这种疾病中的作用，我们评估了MS患者和健康个体中NEST、RMRP、TH2-LCR、MAFTRR和FLICR的表达。我们检测到病例组和对照组之间RMRP和FLICR的表达存在显著差异。患者中NEST、RMRP、TH2-LCR、MAFTRR和FLICR lncRNAs的表达之间存在显著相关性，但对照组中不存在。RMRP与TH2-LCR之间以及MAFTRR与RMRP之间的相关性最强，相关系数分别为0.69和0.59。ROC曲线分析显示FLICR在区分MS患者和健康对照方面具有适当的效能（AUC值 = 0.84）。MS患者中NEST lncRNA的表达与疾病持续时间呈正相关，但与发病年龄呈负相关。简而言之，我们报告了MS患者中两种与T细胞相关的lncRNAs的失调，并提出FLICR作为这种疾病的一个假定标志物。
