Rasheed Narin A, Abdulrahman Rezheen F, Hussein Nawfal R
Medical Department, Akre Technical Institute, Duhok Polytechnic University, Duhok, Kurdistan Region, Iraq.
Pathology and Microbiology Department, College of Veterinary Medicine, University of Duhok, Duhok, Kurdistan Region, Iraq.
IJID Reg. 2022 Jun 6;4:42-46. doi: 10.1016/j.ijregi.2022.06.003. eCollection 2022 Sep.
The aim of the study was to investigate the genetic relatedness between methicillin-resistant (MRSA) strains isolated from the host community of Duhok City and Syrian refugees based on the partial sequences of the gene, and to investigate the prevalence of SCC and vancomycin resistance.
Thirty MRSA isolates that had previously been detected and characterized were included. PCR was used for SCC typing, vancomycin detection, and amplification of the gene for sequencing.
The MRSA isolates were identical and highly similar to globally published strains, especially human nasal cavity strains. The phylogenetic tree showed that the isolates were grouped into two major groups: groups 1 and 2. Group 2 was divergent from group 1 and included isolates from the host community and Syrian refugees (new arrivals). Group 1 included isolates from the three groups, and these were genetically closely related. Most strains in both study groups were SCC type IVa and clustered on the same lineage of the tree. The highest rate of vancomycin resistance was found among the isolates from refugees.
The close relationship between MRSA strains in the two study groups could be due to the geographical location of neighboring countries, which enhances the spread of MRSA strains between them.
本研究旨在基于基因的部分序列，调查从杜胡克市当地社区和叙利亚难民中分离出的耐甲氧西林金黄色葡萄球菌（MRSA）菌株之间的遗传相关性，并调查葡萄球菌盒式染色体（SCC）和万古霉素耐药性的流行情况。
纳入30株先前已检测和鉴定的MRSA分离株。采用聚合酶链反应（PCR）进行SCC分型、万古霉素检测以及用于测序的基因扩增。
MRSA分离株与全球公布的菌株相同且高度相似，尤其是人类鼻腔菌株。系统发育树显示，分离株分为两个主要组：第1组和第2组。第2组与第1组不同，包括来自当地社区和叙利亚难民（新抵达者）的分离株。第1组包括来自这三个组的分离株，它们在基因上密切相关。两个研究组中的大多数菌株为SCC IVa型，并聚集在树的同一谱系上。在难民分离株中发现的万古霉素耐药率最高。
两个研究组中MRSA菌株之间的密切关系可能归因于邻国的地理位置，这促进了MRSA菌株在它们之间的传播。
