Department of Pediatric Surgery, Second Affiliated Hospital of Shantou University Medical College, Shantou, China.
Department of Neonatology, Second Affiliated Hospital of Shantou University Medical College, Shantou, China.
Biomed Chromatogr. 2023 Jan;37(1):e5505. doi: 10.1002/bmc.5505. Epub 2022 Sep 25.
Preterm birth and enteral feeding are two main factors leading to necrotizing enterocolitis (NEC). The metabolomics of preterm infants before and after feeding can provide a basis for the prediction of NEC. Using the method of cross-sectional study, the mode was established with the serum samples of 19 premature infants at birth and after feeding as the control group. The serum was analyzed using GC-MS. Chemometric analysis includes principal component analysis, partial least squares-discriminant analysis, and orthogonal partial least squares-discriminant analysis. Spectral separation of serum metabolites occurred in premature infants before and after feeding. The levels of xylose, d-talose, phosphoglycolic acid, maleimide, l-gulonolactone, maleic acid, β-hydroxypyruvate, itaconic acid, and pantothenic acid in the serum of premature infants after feeding were significant in both multidimensional and single-dimensional modes (variable importance in projection >2, P < 0.01). There was a moderate correlation between total bilirubin and l-gulonolactone and β-hydroxypyruvate (0.8 > r > 0.5). Maleimide, maleic acid, and itaconic acid have diagnostic value (area under the curve >0.9). The results indicated that serum metabolism of preterm infants changes significantly after feeding. Some metabolites have potential value in predicting NEC.
早产和肠内喂养是导致坏死性小肠结肠炎（NEC）的两个主要因素。早产儿喂养前后的代谢组学可以为 NEC 的预测提供依据。本研究采用横断面研究方法，以 19 例早产儿出生时和喂养后的血清样本为对照组，采用 GC-MS 进行血清分析。化学计量学分析包括主成分分析、偏最小二乘判别分析和正交偏最小二乘判别分析。结果显示，早产儿喂养前后血清代谢物发生谱分离。喂养后早产儿血清中木糖、d-塔洛糖、磷酸甘醇酸、马来酰亚胺、l-古洛糖酸内酯、马来酸、β-羟丙酮酸、衣康酸和泛酸的水平在多维和单维模式下均有显著差异（投影变量重要性>2，P<0.01）。总胆红素与 l-古洛糖酸内酯和β-羟丙酮酸之间存在中度相关性（0.8>r>0.5）。马来酰亚胺、马来酸和衣康酸具有诊断价值（曲线下面积>0.9）。结果表明，早产儿喂养后血清代谢明显改变，部分代谢物在预测 NEC 方面具有潜在价值。
