Fayezi Shabnam, Fayyazpour Parisa, Norouzi Zahra, Mehdizadeh Amir
Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Gynecologic Endocrinology and Fertility Disorders, Women's Hospital, Ruprecht-Karls University of Heidelberg, Heidelberg, Germany.
Cell J. 2022 Aug 28;24(8):434-441. doi: 10.22074/cellj.2022.8087.
Primordial germ cells develop into oocytes and sperm cells. These cells are useful resources in reproductive biology and regenerative medicine. The mesenchymal stem cells (MSCs) have been examined for in vitro production of primordial germ cell-like cells. This study aimed to summarize the existing protocols for MSCs differentiation into primordial germ cell-like cells (PGLCs). In the limited identified studies, various models of mesenchymal stem cells, including those derived from adipose tissue, bone marrow, and Wharton's jelly, have been successfully differentiated into primordial germ cell-like cells. Although the protocols of specification induction are basically very similar, they have been adjusted to the mesenchymal cell type and the species of origin. The availability of MSCs has made it possible to customize conditions for their differentiation into primordial germ cell-like cells in several models, including humans. Refining germ cell-related signaling pathways during induced differentiation of MSCs will help define extension to the protocols for primordial germ cell-like cells production.
原始生殖细胞发育成卵母细胞和精子细胞。这些细胞是生殖生物学和再生医学中的有用资源。间充质干细胞（MSCs）已被研究用于体外产生原始生殖细胞样细胞。本研究旨在总结将MSCs分化为原始生殖细胞样细胞（PGLCs）的现有方案。在有限的已鉴定研究中，各种间充质干细胞模型，包括那些源自脂肪组织、骨髓和脐带华通氏胶的模型，已成功分化为原始生殖细胞样细胞。尽管定向诱导方案基本非常相似，但它们已根据间充质细胞类型和来源物种进行了调整。MSCs的可用性使得在包括人类在内的几种模型中定制其分化为原始生殖细胞样细胞的条件成为可能。在MSCs诱导分化过程中优化生殖细胞相关信号通路将有助于确定原始生殖细胞样细胞生产方案的扩展。
