The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
Pediatric Hematology Oncology, Connecticut Children's, Hartford, CT, USA.
Methods Mol Biol. 2023;2677:259-267. doi: 10.1007/978-1-0716-3259-8_15.
Primordial germ cells (PGCs) are the earliest form of mammalian germ lineage. In humans, PGCs are present during a very early and limited window in development, limiting the ability to study fundamental developmental steps in human reproductive biology. However, recent advancements in generating in-vitro models of gametogenesis have allowed the field to generate human primordial germ cell-like cells (hPGCLCs). In this chapter, we will review the generation of hPGCLCs using the incipient mesoderm-like cell (iMeLC) protocol and the subsequent expansion of hPGCLCs in a long-term culture system.
原始生殖细胞 (PGC) 是哺乳动物生殖系的最早形式。在人类中，PGC 存在于发育的非常早期和有限的窗口期，这限制了研究人类生殖生物学中基本发育步骤的能力。然而，最近在配子发生体外模型生成方面的进展使得该领域能够生成人类原始生殖细胞样细胞 (hPGCLC)。在本章中，我们将回顾使用初始中胚层样细胞 (iMeLC) 方案生成 hPGCLC 的过程，以及随后在长期培养系统中扩增 hPGCLC。
