Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, UNITED KINGDOM.
School of Sport, Exercise and Health Sciences (SSEHS) Loughborough University, Loughborough, UNITED KINGDOM.
Med Sci Sports Exerc. 2023 Feb 1;55(2):289-300. doi: 10.1249/MSS.0000000000003040. Epub 2022 Sep 9.
This study aimed to test the hypothesis that a novel nutritional blend composed of tryptophan, glycine, magnesium, tart cherry powder, and l -theanine enhances subjective and objective measures of sleep during free living conditions.
In a randomized, repeated-measures crossover and double-blind deception design, participants ( n = 9 males and 7 females, age = 24 ± 3 yr, body mass = 69.8 ± 11.6 kg, stature = 170.8 ± 9.1 cm) completed a 3-d familiarization period, followed by 3-d intervention and placebo trials. Subjective Pittsburgh Quality Sleep Index, Core Consensus Sleep Diary, and Karolinska Sleepiness Scale survey tools, alongside objective actigraphy measures of sleep, were assessed, with daily nutritional intake, activity, and light exposure standardized between trials. Participants provided daily urine samples for assessment of targeted and untargeted metabolomes.
The intervention trial reduced sleep onset latency (-24 ± 25 min; P = 0.002), increased total sleep time (22 ± 32 min; P = 0.01), and increased sleep efficiency (2.4% ± 3.9%; P = 0.03), while also reducing morning sleepiness ( P = 0.02). Throughout the study, 75% of participants remained blinded to sleep assessment as a primary outcome measure, with 56% subjectively indicating improved sleep during the intervention trial. Metabolomic analysis highlighted several significantly altered metabolomes related to sleep regulation between trials, inclusive of 6-sulfatoxymelatonin, d -serine, and l -glutamic acid.
Data demonstrate that using the proposed blend of novel nutritional ingredients during free living conditions reduced sleep onset latency, increased total sleep duration, and increased sleep efficiency, leading to reduced perceptions of morning sleepiness. These effects may be mediated by the upregulation of key metabolites involved in the neurophysiological modulation of the sleep/wake cycle.
本研究旨在检验一种新型营养混合物（由色氨酸、甘氨酸、镁、酸樱桃粉和 l-茶氨酸组成）是否能在自由生活条件下提高睡眠的主观和客观测量结果的假设。
在随机、重复测量交叉和双盲欺骗设计中，参与者（n=9 名男性和 7 名女性，年龄=24±3 岁，体重=69.8±11.6kg，身高=170.8±9.1cm）完成了 3 天的熟悉期，随后进行了 3 天的干预和安慰剂试验。使用主观匹兹堡睡眠质量指数、核心共识睡眠日记和 Karolinska 嗜睡量表调查工具，以及客观的活动记录仪测量睡眠，在试验之间标准化了每日营养摄入、活动和光照暴露。参与者提供了每日尿液样本，以评估靶向和非靶向代谢组学。
干预试验减少了睡眠潜伏期（-24±25 分钟；P=0.002），增加了总睡眠时间（22±32 分钟；P=0.01），并提高了睡眠效率（2.4%±3.9%；P=0.03），同时也减少了早晨嗜睡（P=0.02）。在整个研究过程中，75%的参与者对睡眠评估作为主要结果指标保持盲法，56%的参与者主观上表示在干预试验期间睡眠得到了改善。代谢组学分析突出了几个在试验之间与睡眠调节相关的显著改变的代谢组学，包括 6-硫酸褪黑素、d-丝氨酸和 l-谷氨酸。
数据表明，在自由生活条件下使用拟议的新型营养成分混合物可以减少睡眠潜伏期，增加总睡眠时间，提高睡眠效率，从而减少早晨嗜睡的感觉。这些效果可能是通过上调参与睡眠/觉醒周期神经生理调节的关键代谢物来介导的。
