Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
Food College, Northeast Agricultural University, Harbin 150030, China.
J Agric Food Chem. 2022 Sep 21;70(37):11678-11688. doi: 10.1021/acs.jafc.2c02909. Epub 2022 Sep 12.
Bifidobacteria are important mediators of immune system development within the gastrointestinal system and immunological homeostasis. The present study explored the anti-colitic activity of H3-R2 in a murine dextran sulfate sodium (DSS)-induced model of ulcerative colitis (UC). Moreover, this study offers novel insight regarding the molecular basis for the probiotic properties of H3-R2 by analyzing the underlying mechanisms whereby H3-R2-derived proteins affect the intestinal barrier. H3-R2 administration was sufficient to alleviate clinical manifestations consistent with DSS-induced colitis, restoring aberrant inflammatory cytokine production, enhancing tight junction protein expression, and positively impacting overall intestinal microecological homeostasis in these animals. Moreover, the bifidobacteria-derived GroEL and transaldolase (TAL) proteins were found to regulate tight junction protein expression via the NF-κB, myosin light chain kinase (MLCK), RhoA/Rho-associated protein kinase (ROCK), and mitogen-activated protein kinase (MAPK) signaling pathways, preventing the lipopolysaccharide (LPS)-mediated disruption of the intestinal epithelial cell barrier.
双歧杆菌是胃肠道系统中免疫系统发育和免疫稳态的重要调节者。本研究探讨了 H3-R2 在葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）小鼠模型中的抗结肠炎活性。此外，通过分析 H3-R2 衍生蛋白影响肠道屏障的潜在机制，本研究为 H3-R2 的益生菌特性提供了新的见解。H3-R2 的给药足以缓解与 DSS 诱导的结肠炎一致的临床表现，恢复异常的炎症细胞因子产生，增强紧密连接蛋白的表达，并对这些动物的整体肠道微生态平衡产生积极影响。此外，双歧杆菌衍生的 GroEL 和转醛醇酶（TAL）蛋白被发现通过 NF-κB、肌球蛋白轻链激酶（MLCK）、RhoA/Rho 相关蛋白激酶（ROCK）和丝裂原激活蛋白激酶（MAPK）信号通路调节紧密连接蛋白的表达，防止脂多糖（LPS）介导的肠上皮细胞屏障破坏。
