College of Biology and the Environment, Nanjing Forestry University, No.159 Longpan Road, Nanjing 210037, Jiangsu Province, Peoples Republic of China.
Zootaxa. 2022 Aug 9;5174(2):165-180. doi: 10.11646/zootaxa.5174.2.3.
We determined the complete mitochondrial genome of Thereuopoda clunifera (Chilopoda: Scutigeridae) to clarify the phylogenetic status within Chilopoda. Using Sanger sequencing and next-generation sequencing technologies, the entire mitogenome of T. clunifera was assembled and annotated, with 14,898 bp in length and 37 genes (13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes), which are usually found in arthropod mitogenomes. Only one D-loop contained no repeat element. The base composition of T. clunifera was found to be A + T content of 69.55% and G + C content of 30.45%. The AT-skew of T. clunifera was positive, while the GC-skew was negative. The gene order of T. clunifera was identical to that of Scutigera coleoptrata that has been unique among those so far determined for the Arthropoda. We also performed phylogenetic analyses of 25 Myriapoda species to further explore the taxonomic and evolutionary relationships within Myriapoda and Chilopoda. Phylogenetic analyses supported that the division of Chilopoda into subclasses Notostigmophora and Pleurostigmophora.
我们测定了栉角蜈蚣（多足纲：地蜈蚣科）的完整线粒体基因组，以阐明多足纲内的系统发育地位。使用 Sanger 测序和下一代测序技术，组装并注释了 T. clunifera 的整个线粒体基因组，长度为 14898bp，包含 37 个基因（13 个蛋白编码基因、22 个转移 RNA 基因和 2 个核糖体 RNA 基因），这些基因通常存在于节肢动物的线粒体基因组中。只有一个 D 环没有重复元件。T. clunifera 的碱基组成发现为 A+T 含量为 69.55%，G+C 含量为 30.45%。T. clunifera 的 AT 偏斜为正，而 GC 偏斜为负。T. clunifera 的基因顺序与 Scutigera coleoptrata 相同，这在迄今为止确定的节肢动物中是独一无二的。我们还对 25 种多足类物种进行了系统发育分析，以进一步探讨多足类和地蜈蚣类内部的分类和进化关系。系统发育分析支持地蜈蚣类分为 Notostigmophora 和 Pleurostigmophora 两个亚纲。
