School of Engineering, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
School of Engineering, University of Guelph, Guelph, Ontario N1G 2W1, Canada; Shrimp Canada, 67 Watson Rd. S (Unit - 2), Guelph, Ontario N1L 1 E3, Canada.
Food Chem. 2023 Feb 1;401:134120. doi: 10.1016/j.foodchem.2022.134120. Epub 2022 Sep 7.
Proteins of low-value and underexplored corn distillers solubles (CDS) have not been considerably valorized. Hence, the influence of one-step enzymatic hydrolysis of proteins with alcalase (A), trypsin (T) or flavourzyme (F) and two steps with AT, TA, AF, FA, TF, or FT was assessed to release peptides with angiotensin-I converting enzyme inhibition (ACEi) and dipeptidyl peptidase4 inhibition (DPP4i). The AF hydrolysate was the best sample in terms of yield, protein content, degree of hydrolysis, ACEi (97.68 ± 1.09 %), and DPP4i (51.51 ± 0.28 %). Mass spectrometry of the most active AF hydrolysate (<3 kDa) identified new major peptides like APLA, PLFP, LFLP, LPPYL, PLYPLP, NDWHTGPL, LPPYLPS, GSPFLGQ, SWQQPIVGG. Bioinformatic analysis showed these can inhibit both ACE and DPP4. This is because peptides contain functional groups and adopt conformations significantly binding with other functional groups at enzyme active sites (p < 0.05). This establishes dual bioactivity of peptides, which may have applications in food, feed, and pharmaceutical industries.
低价值和探索不足的玉米酒糟可溶物（CDS）中的蛋白质尚未得到充分利用。因此，评估了一步法用碱性蛋白酶（A）、胰蛋白酶（T）或风味蛋白酶（F）水解蛋白质，以及两步法用 AT、TA、AF、FA、TF 或 FT 水解蛋白质，以释放具有血管紧张素转化酶抑制（ACEi）和二肽基肽酶 4 抑制（DPP4i）活性的肽。在产率、蛋白质含量、水解度、ACEi（97.68 ± 1.09%）和 DPP4i（51.51 ± 0.28%）方面，AF 水解物是最好的样品。对最活跃的 AF 水解物（<3 kDa）进行质谱分析，鉴定出了新的主要肽类，如 APLA、PLFP、LFLP、LPPYL、PLYPLP、NDWHTGPL、LPPYLPS、GSPFLGQ、SWQQPIVGG。生物信息学分析表明，这些肽可以同时抑制 ACE 和 DPP4。这是因为肽含有功能基团，并采用与酶活性位点上其他功能基团结合的构象（p < 0.05）。这确立了肽的双重生物活性，它可能在食品、饲料和制药行业中有应用。
