Mirzaee Homaira, Ahmadi Gavlighi Hassan, Nikoo Mehdi, Udenigwe Chibuike C, Rezvankhah Amir, Khodaiyan Faramarz
Department of Food Science and Technology, Faculty of Agriculture Tarbiat Modares University Tehran Iran.
Halal Research Center of IRI, Iran Food and Drug Administration Ministry of Health and Medical Education Tehran Iran.
Food Sci Nutr. 2024 Oct 23;12(11):9749-9763. doi: 10.1002/fsn3.4532. eCollection 2024 Nov.
Bioactive peptides and protein hydrolysates have gained considerable attention in the food industry and functional food markets due to their diverse health effects, including antioxidant, antihypertensive, and antidiabetic properties. This study aimed to produce combined soy and corn protein hydrolysates using Alcalase (Al), modification of Al-hydrolysates through sequential hydrolysis using Flavourzyme (Al-FL), cross-linking of Al-hydrolysates using microbial transglutaminase (MTGase) (Al-TG), and fractionation of Al-hydrolysates by ultrafiltration (UF) with molecular weight (MW) cut-off of 100 (Al-F4), 30 (Al-F3), 10 (Al-F2), and 2 kDa (Al-F1). Notably, the < 2 kDa fraction (Al-F1) showcased exceptional biological activities, including antioxidant (81.54% DPPH, 98.02% ABTS), antihypertensive (95.45%), and antidiabetic effects (44.72% α-glucosidase, 77.52% α-amylase), linked to its high hydrophobic amino acid content and low molecular weights (111 and 263 Da). Conversely, the higher molecular weight fraction (Al-TG) excelled in emulsion and foam stability, attributed to its balanced amino acid profile and larger peptides (1385-7057 Da). Our findings reveal that specific protein hydrolysate fractions, particularly Al-F1 and Al-TG, are promising for applications in food and pharmaceutical formulations due to their enhanced biological and functional properties.
生物活性肽和蛋白质水解产物因其多样的健康功效，包括抗氧化、抗高血压和抗糖尿病特性，在食品工业和功能性食品市场中受到了广泛关注。本研究旨在使用碱性蛋白酶（Al）制备大豆和玉米蛋白的复合水解产物，通过使用风味酶进行顺序水解对Al水解产物进行改性（Al-FL），使用微生物转谷氨酰胺酶（MTGase）对Al水解产物进行交联（Al-TG），并通过截留分子量为100（Al-F4）、30（Al-F3）、10（Al-F2）和2 kDa（Al-F1）的超滤（UF）对Al水解产物进行分级分离。值得注意的是，<2 kDa级分（Al-F1）表现出卓越的生物活性，包括抗氧化（DPPH清除率81.54%，ABTS清除率98.02%）、抗高血压（95.45%）和抗糖尿病作用（α-葡萄糖苷酶抑制率44.72%，α-淀粉酶抑制率77.52%），这与其高疏水氨基酸含量和低分子量（111和263 Da）有关。相反，较高分子量级分（Al-TG）在乳液和泡沫稳定性方面表现出色，这归因于其平衡的氨基酸组成和较大的肽（1385 - 7057 Da）。我们的研究结果表明，特定的蛋白质水解产物级分，特别是Al-F1和Al-TG，因其增强的生物学和功能特性，在食品和药物制剂应用中具有广阔前景。
