College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Food Chem. 2023 Feb 1;401:134053. doi: 10.1016/j.foodchem.2022.134053. Epub 2022 Aug 29.
Constructing robust group-specific probes and non-competitive analysis methods for small molecules in the fields of food analysis is of great significance. In this study, three diarrhetic shellfish poisons (DSPs) with high safety risks to humans were taken as models of group targets, and a non-competitive and turn-on format colorimetric aptasensor was constructed for simultaneous and highly sensitive detection of the class of DSPs. A pair of anti-DSP split aptamers were generated with group-specific binding affinity to the three targets. The split aptamers were then fabricated with a hybridization chain reaction (HCR)-enhanced AuNPs nanozyme. The aptasensor realized linear detection range of 187.5-3000 pM, high sensitivity with a limit of detection (LOD) as low as 65.36 pM, good selectivity, as well as good accuracy when analyzing the DSPs in shellfish samples. This study provides good reference for developing robust probes and facile biosensors to detect multiple small molecules in food matrix.
构建用于食品分析领域中小分子的稳健组特异性探针和非竞争分析方法具有重要意义。在本研究中，以三种对人类具有高安全风险的麻痹性贝类毒素（DSPs）为模型靶标，构建了一种非竞争和开启格式的比色适体传感器，用于同时和高灵敏度检测该类 DSPs。生成了一对具有与三个靶标特异性结合亲和力的抗 DSP 分裂适体。然后，将分裂适体与杂交链式反应（HCR）增强的金纳米颗粒纳米酶结合。该适体传感器实现了线性检测范围为 187.5-3000 pM，灵敏度高，检测限（LOD）低至 65.36 pM，具有良好的选择性，以及在贝类样品中分析 DSPs 时的良好准确性。本研究为开发用于检测食品基质中多种小分子的稳健探针和简便生物传感器提供了良好的参考。
