Zhou Zhaoyang, Chen Xingyue, Jiang Shuang, Chen Zhuoer, Wang Sixian, Ren Yueyang, Fan Xiaodong, Le Tao
Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China.
Biosensors (Basel). 2025 Jan 8;15(1):30. doi: 10.3390/bios15010030.
Sulfaquinoxaline (SQX) is widely utilized in aquaculture and animal husbandry due to its broad antimicrobial spectrum and low cost. However, it is difficult to degrade, and there are relevant residues in the aquatic environment, which could be harmful to both the ecological environment and human health. As a new recognition molecule, the aptamer can be recognized with SQX with high affinity and specificity, and the aptamer is no longer adsorbed to AuNPs after binding to SQX, which weakens the catalytic effect of AuNPs. Consequently, an aptasensor for the detection of SQX was successfully developed. This aptasensor exhibits a linear range of 40-640 ng/mL, with a detection limit of 36.95 ng/mL, demonstrating both sensitivity and selectivity. The recoveries of this aptasensor in water samples ranged from 90 to 109.9%, which was quite in line with high-performance liquid chromatography. These findings suggest that the aptasensor is a valuable tool for detecting SQX in aqueous environmental samples.
磺胺喹恶啉（SQX）因其抗菌谱广且成本低而被广泛应用于水产养殖和畜牧业。然而，它难以降解，在水环境中存在相关残留，这可能对生态环境和人类健康都有害。作为一种新型识别分子，适配体可以与SQX以高亲和力和特异性结合，并且适配体在与SQX结合后不再吸附到金纳米颗粒上，这削弱了金纳米颗粒的催化作用。因此，成功开发了一种用于检测SQX的适配体传感器。该适配体传感器的线性范围为40 - 640 ng/mL，检测限为36.95 ng/mL，具有灵敏度和选择性。该适配体传感器在水样中的回收率为90%至109.9%，与高效液相色谱法相当吻合。这些结果表明，该适配体传感器是检测水环境样品中SQX的有价值工具。
