Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China.
BMC Plant Biol. 2022 Sep 13;22(1):438. doi: 10.1186/s12870-022-03794-4.
Proanthocyanidins (PAs) have always been considered as important medicinal value component. In order to gain insights into the PA biosynthesis regulatory network in I. stachyodes roots, we analyzed the transcriptome of the I. stachyodes in Leaf, Stem, RootI (one-year-old root), and RootII (two-year-old root).
In this study, a total of 110,779 non-redundant unigenes were obtained, of which 63,863 could be functionally annotated. Simultaneously, 75 structural genes that regulate PA biosynthesis were identified, of these 6 structural genes (IsF3'H1, IsANR2, IsLAR2, IsUGT72L1-3, IsMATE2, IsMATE3) may play an important role in the synthesis of PAs in I. stachyodes roots. Furthermore, co-expression network analysis revealed that 34 IsMYBs, 18 IsbHLHs, 15 IsWRKYs, 9 IsMADSs, and 3 IsWIPs hub TFs are potential regulators for PA accumulation. Among them, IsMYB24 and IsMYB79 may be closely involved in the PA biosynthesis in I. stachyodes roots.
The biosynthesis of PAs in I. stachyodes roots is mainly produced by the subsequent pathway of cyanidin. Our work provides new insights into the molecular pathways underlying PA accumulation and enhances our global understanding of transcriptome dynamics throughout different tissues.
原花青素（PAs）一直被认为是重要的药用价值成分。为了深入了解黄精根中 PA 生物合成调控网络，我们分析了黄精叶、茎、根 I（一岁根）和根 II（两岁根）的转录组。
本研究共获得 110779 条非冗余的 unigenes，其中 63863 条可进行功能注释。同时，鉴定出 75 个调控 PA 生物合成的结构基因，其中 6 个结构基因（IsF3'H1、IsANR2、IsLAR2、IsUGT72L1-3、IsMATE2、IsMATE3）可能在黄精根中 PA 合成中发挥重要作用。此外，共表达网络分析表明，34 个 IsMYBs、18 个 IsbHLHs、15 个 IsWRKYs、9 个 IsMADSs 和 3 个 IsWIPs 枢纽 TFs 是 PA 积累的潜在调控因子。其中，IsMYB24 和 IsMYB79 可能与黄精根中 PA 生物合成密切相关。
黄精根中 PAs 的生物合成主要由矢车菊素的后续途径产生。我们的工作为 PA 积累的分子途径提供了新的见解，并增强了我们对不同组织中转录组动态的全面理解。
