Andrology Laboratory of State Hospital Denizli, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Pamukkale University Denizli, Turkey.
Reprod Biomed Online. 2022 Nov;45(5):935-942. doi: 10.1016/j.rbmo.2022.06.018. Epub 2022 Jun 26.
Is there a relationship between embryo quality, pregnancy rates and apoptotic gene expression in cumulus cells of oocytes collected from patients with poor ovarian response and polycystic ovary syndrome?
Fifty infertile couples who underwent assisted reproductive technology treatment were included in the study (Approval date 4 February 2020, number 03). The patients were divided into four group: control (n = 9; 90 oocytes), unexplained infertility (n = 8; 86 oocytes), polycystic ovary syndrome (PCOS) (n = 6; 137 oocytes) and poor ovarian response (POR) (n = 27; 124 oocytes). Cumulus cells were isolated individually from 437 oocytes obtained. Intracytoplasmic sperm injection was undertaken on 365 mature oocytes. The embryos were monitored. Caspase-3, Bax and Bcl-2 gene expressions of the cumulus cells were measured by real-time polymerase chain reaction.
A significant and negative correlation was found between Bax and Bcl-2 expressions of the cumulus cells of poor-quality embryos. The increase in Caspase-3 gene expression in the POR group statistically decreases the pregnancy rates. Fertilization and good-quality embryo development of 365 oocytes whose cumulus cells were examined, however, were not associated with apoptotic gene expression. The Bax/Bcl-2 ratio was found to be significantly lower in cumulus cells of mature oocytes.
Our results demonstrated no significant associations between fertilization, quality embryo development and apoptotic gene expression. Bax expression and the Bax/Bcl-2 ratio are high in immature oocyte cumulus cells has shown us that the apoptotic process may begin when the cumulus-oocyte connection exists.
在卵巢反应不良和多囊卵巢综合征患者采集的卵母细胞的卵丘细胞中，胚胎质量、妊娠率和凋亡基因表达之间是否存在关系？
本研究纳入了 50 对接受辅助生殖技术治疗的不孕夫妇（批准日期为 2020 年 2 月 4 日，编号为 03）。患者分为四组：对照组（n=9；90 个卵母细胞）、不明原因不孕组（n=8；86 个卵母细胞）、多囊卵巢综合征组（PCOS）（n=6；137 个卵母细胞）和卵巢反应不良组（POR）（n=27；124 个卵母细胞）。从 437 个获得的卵母细胞中分别分离出卵丘细胞。对 365 个成熟卵母细胞进行了胞浆内单精子注射。监测胚胎。通过实时聚合酶链反应测量卵丘细胞中 Caspase-3、Bax 和 Bcl-2 基因的表达。
发现卵丘细胞中 Bax 和 Bcl-2 表达呈显著负相关。POR 组 Caspase-3 基因表达增加，妊娠率统计学下降。然而，检查卵丘细胞的 365 个卵母细胞的受精和优质胚胎发育与凋亡基因表达无关。发现成熟卵母细胞的卵丘细胞中 Bax/Bcl-2 比值明显降低。
我们的结果表明，受精、优质胚胎发育与凋亡基因表达之间没有显著相关性。不成熟卵母细胞卵丘细胞中 Bax 表达和 Bax/Bcl-2 比值较高，表明当卵丘-卵母细胞连接存在时，凋亡过程可能开始。
