Swietlik John F, Knott Emily A, Longo Katherine C, Zlevor Annie M, Zhang Xiaofei, Laeseke Paul F, Reeder Scott B, Xu Zhen, Lee Fred T, Ziemlewicz Timothy J
Department of Radiology, University of Wisconsin, 600 Highland Ave, Madison, WI, 53792, USA.
Department of Pathology, University of Wisconsin, 1685 Highland Ave, Madison, WI, 53705, USA.
Cardiovasc Intervent Radiol. 2023 Jan;46(1):120-127. doi: 10.1007/s00270-022-03262-4. Epub 2022 Sep 12.
This study was designed to evaluate the feasibility and safety of histotripsy subcutaneous (SQ) fat treatment in an in-vivo porcine model, and evaluate evolution of the treated volume on MRI and pathology.
METHODS/MATERIALS: 10 histotripsy SQ fat treatments were completed in 5 swine, divided into four groups based on pre-determined survival: day 0 (n = 4), day 7 (n = 2), day 28 (n = 2), and day 56 (n = 2). A 4.0 × 4.0x2.0 cm ovoid treatment was created in the fat pad of the posterior thorax. MRI of survived animals were obtained on day 7 (n = 6), day 28 (n = 4), and day 56 (n = 2), and reviewed for size and imaging characteristics. Technical success was defined as the creation of a treatment zone in the targeted SQ fat. Skin firmness and indentation were qualitatively scored.
Histotripsy had a 100% (10/10) technical success for creation of SQ fat treatments. Mean treatment time was 35.5 min (range 35-36.5). The volume of treated SQ fat demonstrated 92% volume reduction over the study. Day 0 gross pathology treatment had a mean volume of 12.6 cm (± 2.1) (prescribed volume of 16.7 cm), which decreased to 8.3 cm (± 2.8) by day 7 (34% overall decrease), 3.0 cm (± 0.5) by day 28 (76% overall decrease), and 1.0 cm (± 1.2) by day 56 (92% overall decrease). Mean firmness and indentation scores showed no change from baseline at all time points, with no overlying skin injury.
Histotripsy safely and effectively treated SQ fat of an in-vivo porcine model, with volume reduction over time.
本研究旨在评估在猪体内模型中进行组织粉碎皮下（SQ）脂肪治疗的可行性和安全性，并通过磁共振成像（MRI）和病理学评估治疗体积的变化情况。
方法/材料：对5头猪进行了10次组织粉碎皮下脂肪治疗，根据预定存活时间分为四组：第0天（n = 4）、第7天（n = 2）、第28天（n = 2）和第56天（n = 2）。在猪后胸部脂肪垫处创建一个4.0×4.0×2.0 cm的椭圆形治疗区域。对存活动物在第7天（n = 6）、第28天（n = 4）和第56天（n = 2）进行MRI检查，并评估治疗区域的大小和成像特征。技术成功定义为在目标皮下脂肪中创建治疗区域。对皮肤硬度和压痕进行定性评分。
组织粉碎在创建皮下脂肪治疗区域方面技术成功率为100%（10/10）。平均治疗时间为35.5分钟（范围35 - 36.5分钟）。在整个研究过程中，治疗的皮下脂肪体积减少了92%。第0天大体病理学检查显示治疗区域平均体积为12.6 cm³（±2.1）（规定体积为16.7 cm³），到第7天降至8.3 cm³（±2.8）（总体减少34%），到第28天降至3.0 cm³（±0.5）（总体减少76%），到第56天降至1.0 cm³（±1.2）（总体减少92%）。平均硬度和压痕评分在所有时间点与基线相比均无变化，且未出现皮肤损伤。
组织粉碎安全有效地治疗了猪体内模型的皮下脂肪，且随着时间推移体积减小。
