Toktay Halil, Akyol Badel G, Evlice Emre, İmren Mustafa
Department of Plant Production and Technologies, Faculty of Agricultural Sciences and Technologies, University of Nigde, Nigde, Turkey.
Department of Plant Protection, Faculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, 58140, Sivas, Turkey.
Mol Biol Rep. 2022 Dec;49(12):11557-11562. doi: 10.1007/s11033-022-07860-w. Epub 2022 Sep 12.
The aim of this study was to identified cyst nematodes in the cabbage production areas in Niğde Province by molecular methods to underpin decision making for field control. The sequences of ribosomal DNA region (rDNA-ITS) and cytochrome oxidase subunit 1 (mtDNA-COI) were used for the first time for identification Heterodera cruciferae (cabbage cyst nematode) in Turkey.
Heterodera cruciferae populations extracted from cabbage growing areas of Niğde Province and investigated using sequences of rDNA-ITS and mtDNA-COI. Similarities and differences between 13 geographic populations of H. cruciferae were detected in surveys during 2020-2021. DNA from single cysts was successfully amplified, and genetic variability was revealed within nematode populations. Based on these results, H. cruciferae is reported for the first time from Niğde Province, Turkey. This study showed clear discrimination among the sampled populations of H. cruciferae.
This finding is important for control and managing cabbage cyst nematode in cabbage fields in Turkey as more than one Heterodera spp. can occur. Future studies should investigate the population dynamics and control of H. cruciferae in fields in the sampled districts.
本研究旨在通过分子方法鉴定尼德省卷心菜产区的孢囊线虫，为田间防治决策提供依据。核糖体DNA区域（rDNA-ITS）和细胞色素氧化酶亚基1（mtDNA-COI）序列首次用于土耳其十字花科异皮线虫（卷心菜孢囊线虫）的鉴定。
从尼德省卷心菜种植区提取十字花科异皮线虫种群，并利用rDNA-ITS和mtDNA-COI序列进行研究。在2020 - 2021年的调查中检测到13个十字花科异皮线虫地理种群之间的异同。成功扩增了单个孢囊的DNA，并揭示了线虫种群内的遗传变异性。基于这些结果，首次报道了土耳其尼德省存在十字花科异皮线虫。本研究显示了所采样的十字花科异皮线虫种群之间的明显差异。
这一发现对于土耳其卷心菜田防治和管理卷心菜孢囊线虫具有重要意义，因为可能会出现不止一种异皮线虫属物种。未来的研究应调查采样地区田间十字花科异皮线虫的种群动态和防治情况。
