Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, 119071, Moscow, Russia.
Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Kashirskoye shosse 24, 115478, Moscow, Russia.
Anal Bioanal Chem. 2022 Nov;414(26):7553-7563. doi: 10.1007/s00216-022-04298-8. Epub 2022 Sep 13.
Aquatic toxins are a group of toxic compounds produced by several types of freshwater and marine algae and cyanobacteria and transported through the food chains of water bodies. Potential contamination of aquaculture products (raw and processed fish and seafood) with aquatic toxins requires the use of efficient screening methods for their control. In this study, a multiplex immunochromatographic test system for the simultaneous detection of three aquatic toxins-phycotoxins domoic acid (DA) and okadaic acid (OA), and cyanotoxin microcystin-LR (MC-LR)-is for the first time developed. For this, a competitive indirect immunochromatographic analysis (ICA) based on gold-labeled secondary antibodies was carried out. The LODs/cutoffs/working ranges of the ICA were 0.05/0.3/0.07-0.29, 1.3/100/3.2-58.2, and 0.1/2.0/0.2-1.1 ng/mL for MC-LR, DA, and OA, respectively. The assay duration was 18 min. The developed test system was used to analyze water samples from natural sources (salt and fresh water) and fish samples. For sample preparation of water, simple dilution with a buffer was proposed; for fish samples, methanol-water extraction was utilized. It was demonstrated that the triple LFIA specifically detected target aquatic toxins with recoveries of 85.0-121.5%. The developed multiplex LFIA can be considered a promising analytical solution for the rapid, easy, and sensitive control of water and food safety.
水生毒素是一组由几种淡水和海洋藻类及蓝细菌产生的有毒化合物，通过水体食物链进行传输。水产养殖产品（生的和加工的鱼和海鲜）可能受到水生毒素的污染，因此需要使用有效的筛选方法来进行控制。在这项研究中，首次开发了一种用于同时检测三种水生毒素——藻毒素软骨藻酸（DA）和 okadaic 酸（OA）以及蓝藻毒素微囊藻-LR（MC-LR）的多重免疫层析测试系统。为此，进行了基于金标记二抗的竞争性间接免疫层析分析（ICA）。ICA 的 LODs/截止值/工作范围分别为 0.05/0.3/0.07-0.29、1.3/100/3.2-58.2 和 0.1/2.0/0.2-1.1 ng/mL，用于 MC-LR、DA 和 OA。检测时间为 18 分钟。所开发的测试系统用于分析来自天然来源（盐和淡水）的水样和鱼样。对于水样的制备，建议采用缓冲液简单稀释；对于鱼样，采用甲醇-水提取。结果表明，三重 LFIA 特异性检测目标水生毒素，回收率为 85.0-121.5%。所开发的多重 LFIA 可被视为一种用于快速、简便和灵敏控制水和食品安全的有前景的分析解决方案。
