Hendrickson Olga D, Zvereva Elena A, Solopova Olga N, Zherdev Anatoly V, Sveshnikov Peter G, Eremin Sergei A, Dzantiev Boris B
Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Kashirskoye Shosse 24, 115478 Moscow, Russia.
Micromachines (Basel). 2022 Sep 10;13(9):1506. doi: 10.3390/mi13091506.
In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with anti-species antibodies. Under optimal conditions, the instrumental detection limits/cutoffs for simultaneous detection of DA and OA were 1.2/100 and 0.1/2.5 ng/mL, respectively. The time of the assay was 18 min. The ICA was applied to test seawater and a large panel of seafood, including mussels, tiger shrimps, octopuses, whelks, crabs, and scallops. The proposed simple sample preparation method for seafood takes only 20 min. For seawater, a dilution by buffer was implemented. The assay recoveries varied from 80.8% to 124.5%. The competitive potential of the proposed technique as a tool to control natural water and seafood samples is determined by its simplicity, rapidity, and sensitivity.
在本研究中，首次开发了一种用于同时检测两种相关藻毒素——软骨藻酸（DA）和冈田酸（OA）的双免疫层析分析（ICA）方法。该免疫层析分析采用与抗物种抗体偶联的金纳米颗粒以间接竞争模式进行。在最佳条件下，同时检测DA和OA的仪器检测限/临界值分别为1.2/100和0.1/2.5 ng/mL。检测时间为18分钟。该免疫层析分析方法应用于检测海水以及包括贻贝、虎虾、章鱼、蛾螺、螃蟹和扇贝在内的大量海鲜样本。所提出的用于海鲜的简单样品制备方法仅需20分钟。对于海水，采用缓冲液进行稀释。该检测方法的回收率在80.8%至124.5%之间。所提出的技术作为一种用于控制天然水和海鲜样本的工具，其竞争潜力取决于其简便性、快速性和灵敏度。
