Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province, China.
The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu Province, China.
Insect Sci. 2023 Jun;30(3):789-802. doi: 10.1111/1744-7917.13114. Epub 2022 Oct 10.
The silkworm Bombyx mori L. is a model organism of the order Lepidoptera. Understanding the mechanism of pesticide resistance in silkworms is valuable for Lepidopteran pest control. In this study, comparative metabolomics was used to analyze the metabolites of 2 silkworm strains with different pesticide resistance levels at 6, 12, and 24 h after feeding with fenpropathrin. Twenty-six of 27 metabolites showed significant differences after fenpropathrin treatment and were classified into 6 metabolic pathways: glycerophospholipid metabolism, sulfur metabolism, glycolysis, amino acid metabolism, the urea cycle, and the tricarboxylic acid (TCA) cycle. After analyzing the percentage changes in the metabolic pathways at the 3 time points, sulfur metabolism, glycolysis, and the TCA cycle showed significant responses to fenpropathrin. Confirmatory experiments were performed by feeding silkworms with key metabolites of the 3 pathways. The combination of iron(II) fumarate + folic acid (IF-FA) enhanced fenpropathrin resistance in silkworms 6.38 fold, indicating that the TCA cycle is the core pathway associated with resistance. Furthermore, the disruption of several energy-related metabolic pathways caused by fenpropathrin was shown to be recovered by IF-FA in vitro. Therefore, IF-FA may have a role in boosting silkworm pesticide resistance by modulating the equilibrium between the TCA cycle and its related metabolic pathways.
家蚕（Bombyx mori L.）是鳞翅目昆虫的模式生物。了解家蚕对杀虫剂产生抗药性的机制对于鳞翅目害虫的防治具有重要意义。在本研究中，采用比较代谢组学方法分析了 2 个对杀虫剂具有不同抗性水平的家蚕品系在喂食高效氯氟氰菊酯后 6、12 和 24 h 的代谢物。在高效氯氟氰菊酯处理后，27 种代谢物中有 26 种表现出显著差异，并被分为 6 种代谢途径：甘油磷脂代谢、硫代谢、糖酵解、氨基酸代谢、尿素循环和三羧酸（TCA）循环。分析 3 个时间点的代谢途径百分比变化后，硫代谢、糖酵解和 TCA 循环对高效氯氟氰菊酯表现出显著的响应。通过对 3 条途径的关键代谢物进行家蚕喂食验证实验。富马酸亚铁（iron(II) fumarate）+叶酸（folic acid）（IF-FA）的组合使家蚕对高效氯氟氰菊酯的抗性增强了 6.38 倍，表明 TCA 循环是与抗性相关的核心途径。此外，高效氯氟氰菊酯导致的几种与能量相关的代谢途径的破坏在体外被 IF-FA 恢复。因此，IF-FA 可能通过调节 TCA 循环及其相关代谢途径之间的平衡，在家蚕对杀虫剂的抗性增强中发挥作用。
