Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, Jiangsu, China.
Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, Jiangsu, China; The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang 212100, Jiangsu, China.
Pestic Biochem Physiol. 2023 Aug;194:105485. doi: 10.1016/j.pestbp.2023.105485. Epub 2023 Jun 3.
The widespread use of pyrethroid pesticides has brought serious economic losses in sericulture, but there is still no viable solution. The key to solving the problem is to improve silkworm resistance to pesticides, which depends on understanding the resistance mechanism of silkworms to pesticides. This study aimed to use transcriptomes to understand the underlying mechanism of silkworm resistance to fenpropathrin, which will provide a theoretical molecular reference for breeding pesticide-resistant silkworm varieties. In this study, the fat bodies of two strains with differential resistance after 12 h of fenpropathrin feeding were analyzed using RNA-Seq. After feeding fenpropathrin, 760 differentially expressed genes (DEGs) were obtained in the p50(r) strain and 671 DEGs in the 8y strain. The DEGs involved in resistance to fenpropathrin were further identified by comparing the two strains, including 207 upregulated DEGs in p50(r) and 175 downregulated DEGs in 8y. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that these fenpropathrin-related DEGs are mainly enriched in the metabolism and transporter pathways. Moreover, 28 DEGs involved in the metabolic pathway and 18 in the transporter pathway were identified. Furthermore, organic cation transporter protein 6 (BmOCT6), a transporter pathway member, was crucial in enhancing the tolerance of BmN cells to fenpropathrin. Finally, the knockdown of the expression of the homologs of BmOCT6 in Glyphodes pyloalis (G. pyloalis) significantly decreased the resistant level of larvae to fenpropathrin. The findings showed that the metabolism and transporter pathways are associated with resistance to fenpropathrin in silkworm, and OCT6 is an effective and potential target not only for silkworm breeding but also for pest biocontrol.
拟除虫菊酯类农药的广泛使用给蚕桑业带来了严重的经济损失，但目前仍没有可行的解决方案。解决这一问题的关键在于提高家蚕对农药的抗性，这取决于对家蚕对农药抗性机制的理解。本研究旨在利用转录组学来了解家蚕对甲氰菊酯抗性的潜在机制，这将为培育抗农药家蚕品种提供理论分子参考。在这项研究中，我们通过 RNA-Seq 分析了两种在甲氰菊酯喂食 12 小时后具有不同抗性的家蚕的脂肪体。在喂食甲氰菊酯后，在 p50(r)菌株中获得了 760 个差异表达基因 (DEGs)，在 8y 菌株中获得了 671 个 DEGs。通过比较这两个菌株，进一步鉴定了与甲氰菊酯抗性相关的 DEGs，包括在 p50(r)中上调的 207 个 DEGs 和在 8y 中下调的 175 个 DEGs。基因本体论 (GO) 和京都基因与基因组百科全书 (KEGG) 富集分析表明，这些与甲氰菊酯相关的 DEGs 主要富集在代谢和转运途径中。此外，还鉴定了 28 个参与代谢途径和 18 个参与转运途径的 DEGs。此外，有机阳离子转运蛋白 6 (BmOCT6)，一种转运途径成员，对增强 BmN 细胞对甲氰菊酯的耐受性至关重要。最后，敲低 G. pyloalis 同源物的表达显著降低了幼虫对甲氰菊酯的抗性水平。研究结果表明，代谢和转运途径与家蚕对甲氰菊酯的抗性有关，OCT6 不仅是家蚕育种的有效和潜在靶点，也是害虫生物防治的有效和潜在靶点。
