Perrone Maria Rita, Romano Salvatore, De Maria Giuseppe, Tundo Paolo, Bruno Anna Rita, Tagliaferro Luigi, Maffia Michele, Fragola Mattia
Department of Mathematics and Physics, University of Salento, 73100 Lecce, Italy.
Presidio Ospedaliero Santa Caterina Novella, Azienda Sanitaria Locale Lecce, 73013 Galatina, Lecce, Italy.
Aerobiologia (Bologna). 2022;38(3):391-412. doi: 10.1007/s10453-022-09754-7. Epub 2022 Sep 8.
The SARS-CoV-2 presence and the bacterial community profile in air samples collected at the Intensive Care Unit (ICU) of the Operational Unit of Infectious Diseases of Santa Caterina Novella Hospital in Galatina (Lecce, Italy) have been evaluated in this study. Air samplings were performed in different rooms of the ICU ward with and without COVID-19 patients. No sample was found positive to SARS-CoV-2, according to Allplex 2019-nCoV Assay. The airborne bacterial community profiles determined by the 16S rRNA gene metabarcoding approach up to the species level were characterized by richness and biodiversity indices, Spearman correlation coefficients, and Principal Coordinate Analysis. Pathogenic and non-pathogenic bacterial species, also detected in outdoor air samples, were found in all collected indoor samples. , and others , detected at high relative abundances in all the patients' rooms, were the most abundant pathogenic species. The highest mean relative abundance of and suggested that they were likely the main pathogens of COVID-19 patients at the ICU ward of this study. The identification of nosocomial pathogens representing potential patients' risks in ICU COVID-19 rooms and the still controversial airborne transmission of the SARS-CoV-2 are the main contributions of this study.
The online version contains supplementary material available at 10.1007/s10453-022-09754-7.
本研究评估了在意大利莱切省加拉蒂纳市圣卡特琳娜诺维拉医院传染病科重症监护病房（ICU）采集的空气样本中SARS-CoV-2的存在情况和细菌群落特征。在ICU病房有COVID-19患者和无COVID-19患者的不同房间进行了空气采样。根据Allplex 2019-nCoV检测，未发现样本对SARS-CoV-2呈阳性。通过16S rRNA基因元条形码方法确定的空气传播细菌群落特征，直至物种水平，通过丰富度和生物多样性指数、斯皮尔曼相关系数和主坐标分析进行表征。在所有采集的室内样本中都发现了在室外空气样本中也检测到的致病和非致病细菌物种。在所有患者房间中相对丰度较高的[具体物种1]、[具体物种2]等是最丰富的致病物种。[具体物种3]和[具体物种4]的最高平均相对丰度表明，它们可能是本研究中ICU病房COVID-19患者的主要病原体。识别代表ICU COVID-19病房潜在患者风险的医院病原体以及SARS-CoV-2仍存在争议的空气传播是本研究的主要贡献。
在线版本包含可在10.1007/s10453-022-09754-7获取的补充材料。
