Lund-Andersen H, Larsen M, Dalgaard P, Olsen W
Graefes Arch Clin Exp Ophthalmol. 1987;225(3):173-6. doi: 10.1007/BF02175445.
Fluorescein (F) and fluorescein glucuronide (FG) were determined in the vitreous of four diabetic patients by a double-filter slit-lamp fluorophotometric technique. Determinations were performed 60-80 min after i.v. injection of fluorescein. F and FG were also determined in plasma ultrafiltrate 5, 15, 30, 60 and 120 min after injection by high-pressure liquid chromatography. The concentration of FG in the vitreous was 3 times that of F. After correction for plasma concentrations of FG higher than those of F, the penetration index of FG through the blood-retinal barrier was found to be twice the penetration index of F. This is not what would be expected if passive transport alone were involved. Accordingly, it is suggested that active transport mechanisms contribute to the movement of F and FG across the blood-retinal barrier.
采用双滤光裂隙灯荧光光度法测定了4例糖尿病患者玻璃体中的荧光素（F）和荧光素葡糖醛酸苷（FG）。在静脉注射荧光素后60 - 80分钟进行测定。还通过高压液相色谱法在注射后5、15、30、60和120分钟测定血浆超滤液中的F和FG。玻璃体中FG的浓度是F的3倍。在校正血浆中FG浓度高于F的浓度后，发现FG穿过血视网膜屏障的通透指数是F的通透指数的两倍。如果仅涉及被动转运，情况并非如此。因此，提示主动转运机制有助于F和FG穿过血视网膜屏障。
