Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.
Anal Chem. 2022 Sep 27;94(38):13278-13286. doi: 10.1021/acs.analchem.2c03393. Epub 2022 Sep 13.
The point-of-care (POC) method with affordability and portability for the sensitive detection of biological substances is an emerging topic in rapid disease screening and personalized medicine. In this work, we demonstrated a diverse responsive platform based on a dual-channel pressure sensor (DCPS). The DCPS had a multilayer flexible architecture consisting of a photonic hydrogel with chromatic transitions and a piezoresistive pressure sensor as the electrical data transmission unit, both of which had the property of pressure-induced mechanical stimulus feedback. By incorporating a platinum nanoparticles-labeled detection antibody (PtNPs-dAb) into the sandwich-type immunoreaction for the target carcinoembryonic antigen (CEA, as a model analyte), gas decomposition could be triggered by the addition of hydrogen peroxide (HO) to induce a significant increase under pressure in a closed chamber. Meanwhile, the DCPS enabled an accurate electrical signal output, and the photonic hydrogel converted spatiotemporal stimuli into eye-readable colorations with string brilliance. In this way, the target concentration could be quantificationally related to the electrical response and intuitively perceived through visible color alterations. Under optimal conditions, a sensitive determination of CEA was performed in a detectable range of 0.3-60 ng/mL with a limit of detection (LOD) of 0.13 ng/mL. In addition, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility. Furthermore, an array-based immunoassay device was fabricated to conceptually validate its application potential in high-throughput biomedical detection and inspire a dual-signal POC diagnostic platform in a friendly way for resource-limited settings.
即时检测（POC）方法具有经济实惠且便于携带的特点，适用于生物物质的灵敏检测，是快速疾病筛查和个性化医疗领域的一个新兴课题。在本工作中，我们展示了一种基于双通道压力传感器（DCPS）的多功能响应平台。该 DCPS 具有多层柔性结构，由具有颜色转变特性的光子水凝胶和作为电数据传输单元的压阻压力传感器组成，两者均具有压力诱导机械刺激反馈的特性。通过将标记有铂纳米粒子的检测抗体（PtNPs-dAb）纳入针对目标癌胚抗原（CEA 的三明治型免疫反应中（作为模型分析物），通过添加过氧化氢（HO）来触发气体分解，在封闭腔内会在压力下引发显著增加。同时，DCPS 能够实现准确的电信号输出，而光子水凝胶则将时空刺激转化为具有闪烁效果的可目视颜色变化。通过这种方式，目标浓度可以与电响应定量相关，并通过可见颜色变化直观感知。在最佳条件下，对 CEA 进行了灵敏检测，检测范围为 0.3-60ng/mL，检测限（LOD）为 0.13ng/mL。此外，该方案具有良好的选择性、准确性和重现性。此外，还构建了基于阵列的免疫分析装置，从概念上验证了其在高通量生物医学检测中的应用潜力，并以友好的方式为资源有限的环境激发了双信号 POC 诊断平台。
