Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Testing Center , Fuzhou University , Fuzhou 35011168 , People's Republic of China.
ACS Sens. 2019 Sep 27;4(9):2272-2276. doi: 10.1021/acssensors.9b01168. Epub 2019 Jul 23.
A saw-toothed microstructure-based flexible pressure sensor was designed as the signal readout for point-of-care (POC) immunoassay of carcinoembryonic antigen (CEA). In this portable POC bioassay, the sandwich-type immunoreaction was first carried out to capture the target on a microplate, which simultaneously introduced the platinum nanoparticles (PtNPs). Upon adding hydrogen peroxide (HO), the pressure as the bridge between the molecular recognition event and detectable signal was increasing rapidly, resulting from the decomposition of HO accelerated by PtNPs. Meanwhile, a flexible pressure sensor was fabricated with high sensitivity; the ability of the pressure response was dramatically improved by adopting the saw-toothed microstructure. By coupling with the pressure sensor, the pressure change could be monitored in real time to achieve the portable detection of CEA. Under the optimum conditions, the proposed pressure-based bioassay presented good sensing performance within 0.1-40 ng/mL at a detection limit of 87 pg/mL. The reproducibility, precision, and accuracy provided by the method were also studied and satisfied.
一种基于锯齿状微观结构的柔性压力传感器被设计为即时检测（POC）癌胚抗原（CEA）免疫分析的信号读取器。在这种便携式即时生物检测中，夹心型免疫反应首先在微孔板上进行，以捕获目标，同时引入铂纳米颗粒（PtNPs）。加入过氧化氢（HO）后，压力作为分子识别事件和可检测信号之间的桥梁迅速增加，这是由于 PtNPs 加速了 HO 的分解。同时，采用锯齿状微观结构制造了具有高灵敏度的柔性压力传感器；压力响应能力通过采用锯齿状微观结构得到了显著提高。通过与压力传感器耦合，可以实时监测压力变化，实现 CEA 的便携式检测。在最佳条件下，所提出的基于压力的生物测定法在检测限为 87pg/mL 时在 0.1-40ng/mL 范围内表现出良好的传感性能。该方法的重现性、精密度和准确性也得到了研究和满足。
