Pak J Biol Sci. 2022 Jun;25(7):602-607. doi: 10.3923/pjbs.2022.602.607.
<b>Background and Objective:</b> <i>Kalanchoe tomentosa</i> is identified and their different characteristics regarding the antibacterial and antioxidant properties have a vast effect. Fresh <i>K. tomentosa</i> leaves obtained from Bandung, Indonesia was extracted using n-hexane followed by serial dichloromethane maceration. <b>Materials and Methods:</b> N-hexane and ethyl acetate were used to separate the dichloromethane extract using vacuum liquid chromatography and the isolated compounds were recrystallized with n-hexane. <b>Results:</b> About 37 mg of dichloromethane extract was obtained from the extraction process. Recrystallized compound isolates were identified as stigmast-5-en-3-ol or β-sitosterol. Both dichloromethane extract and β-sitosterol isolated compounds showed strong bacteriostatic activity against <i>S. aureus</i> with MIC = 15.63 and 7.81 μg mL<sup></sup><sup>1</sup> and<i> K. pneumonia</i> with MIC = 7.81 and 31.25 μg mL<sup></sup><sup>1</sup>, respectively. However, only dichloromethane extract exhibited a bactericidal effect (7.81 μg mL<sup></sup><sup>1</sup>). <b>Conclusion:</b> The pure β-sitosterol compound was isolated from<i> K. tomentosa</i> dichloromethane extract. Both the dichloromethane extract and the isolated β-sitosterol compound had antibacterial effects against <i>S. aureus</i> and <i>K. pneumonia.</i>.
长寿花已被鉴定，其不同特性在抗菌和抗氧化方面具有广泛的影响。从印度尼西亚万隆获得的新鲜长寿花叶片，使用正己烷提取，然后进行连续二氯甲烷浸取。材料与方法：使用正己烷和乙酸乙酯通过真空液-液色谱法分离二氯甲烷提取物，并用正己烷对分离得到的化合物进行重结晶。结果：从提取过程中得到约 37 毫克的二氯甲烷提取物。重结晶化合物分离物被鉴定为豆甾-5-烯-3-醇或β-谷甾醇。二氯甲烷提取物和分离得到的β-谷甾醇化合物均对金黄色葡萄球菌表现出较强的抑菌活性，MIC = 15.63 和 7.81μg/mL；对肺炎克雷伯菌的 MIC = 7.81 和 31.25μg/mL。然而，只有二氯甲烷提取物表现出杀菌作用（7.81μg/mL）。结论：从长寿花的二氯甲烷提取物中分离出纯β-谷甾醇化合物。二氯甲烷提取物和分离得到的β-谷甾醇化合物均对金黄色葡萄球菌和肺炎克雷伯菌具有抗菌作用。
