Pak J Biol Sci. 2022 Jan;25(6):495-500. doi: 10.3923/pjbs.2022.495.500.
<b>Background and Objective:</b> Ginger (<i>Zingiber officinale</i> (Wild). Roscoe) is a plant that has long been used as a medicinal herb for humans, so it has been grown and popularized in the Mekong Delta Provinces, but the research systematic genetics has not been given much attention. The current study evaluated the genetic characteristics of some ginger samples collected in eight provinces in the Mekong Delta based on morphological characteristics and ITS gene sequence regions. <b>Materials and Methods:</b> Samples of Ginger varieties were collected in eight provinces, fresh leaf samples were collected and stored frozen at -20°C. Ginger morphology observation and description based on improved plant research methods. Total DNA extraction of ginger leaf samples was extracted from fresh leaf samples according to the extraction procedure by the modified CTAB method. <b>Results:</b> The phenotypes of the samples like length, leaf width, flower length and fruit diameter were significantly different between growing regions due to different environmental and farming conditions. Genetic relationship analysis showed that there are two distinct groups. Group I has 5 ginger samples from G8-Vinh Long, G4-Ben Tre, G2-Ca Mau, G5-Dong Thap and G6-Hau Giang provinces and group II includes ginger varieties belonging to G7-Ho Chi Minh City, G1-An Giang Province and G3-City. Can Tho. <b>Conclusion:</b> Samples/cultivars collected from eight provinces in the Mekong Delta showed variation in agronomic characteristics, but only stem height and length changes were statistically significantly different. Genotyping most of the samples belonged to the species <i>Zingiber officinale</i> (Wild) Roscoe.
<b>背景与目的:</b>姜(<i>Zingiber officinale</i>(Wild)Roscoe)是一种长期以来一直被用作人类药用植物的植物,因此在湄公河三角洲省份得到了种植和推广,但系统遗传学研究并没有得到太多关注。本研究基于形态特征和 ITS 基因序列区域,对湄公河三角洲八个省份采集的一些姜样本进行了遗传特征评估。<b>材料与方法:</b>在八个省份采集姜品种样本,采集新鲜叶片样本并在-20°C 下冷冻保存。根据改良植物研究方法对姜形态进行观察和描述。根据改良 CTAB 法的提取程序,从新鲜叶片样本中提取姜叶片样本的总 DNA。<b>结果:</b>由于环境和耕作条件的不同,不同种植区的样本表型(如长度、叶宽、花长和果径)差异显著。遗传关系分析表明存在两个明显的群体。第一组有来自 G8-永隆、G4-槟椥、G2-薄寮、G5-同塔和 G6-后江省的 5 个姜样本,第二组包括属于 G7-胡志明市、G1-安江省和 G3-芹苴市的姜品种。<b>结论:</b>湄公河三角洲八个省份采集的样本/品种在农艺性状上表现出变异,但只有茎高和长度的变化具有统计学显著性差异。对大多数样本进行基因型分析,发现它们属于种<i>Zingiber officinale</i>(Wild)Roscoe。