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紫薯( Lam.)废料的潜在抗氧化活性。

Potential Antioxidative Activity of Waste Product of Purple Sweet Potato ( Lam.).

出版信息

Pak J Biol Sci. 2022 Jan;25(8):681-687. doi: 10.3923/pjbs.2022.681.687.

Abstract

<b>Background and Objective:</b> Antioxidants are substances that can deactivate free radicals. Phenol and flavonoid are antioxidant compounds widely found in plants, including purple sweet potato (<i>Ipomoea batatas</i> L.). This research aimed to investigate three purple sweet potato-based organs' antioxidative activity and flavonoid contents. <b>Materials and Methods:</b> Antioxidative activities, total phenolic content and total flavonoid content were performed by UV-visible spectrophotometry. Pearson's method analyzed the correlation of TPC and TFC with antioxidative activities and the correlation between two antioxidative testing methods. <b>Results:</b> Antioxidative activity of three organs purple sweet potato using DPPH method showed values varied from 6.572-290.894 mg AAE g<sup>1</sup> and using CUPRAC method varied from 25.169-621.254 mg AAE g<sup>1</sup>. The highest TPC was found in ethanolic leaves extract (20.885 g GAE 100 g<sup>1</sup>), while the highest TFC was found in ethyl acetate leaves extract (10.048 g QE 100 g<sup>1</sup>). <b>Conclusion:</b> DPPH and CUPRAC tests revealed that purple sweet potato leaves, stem and tuber extracts were potent antioxidants. The potential antioxidative activity was found in the waste product of purple sweet potatoes (leaves and stem). Phenol and flavonoid compounds had contributors to antioxidative activity. DPPH and CUPRAC methods gave linear results for most of the antioxidative activity in three organs of purple sweet potato. Ethanol stem extract contained luteolin 7-O-glucoside, rutin, quercetin, kaempferol and apigenin. Rutin had the highest content, which was 0.399%.

摘要

背景与目的

抗氧化剂是能使自由基失活的物质。酚类和类黄酮是广泛存在于植物中的抗氧化化合物,包括紫甘薯(Ipomoea batatas L.)。本研究旨在探讨三种紫甘薯器官的抗氧化活性和类黄酮含量。

材料与方法

采用紫外可见分光光度法测定抗氧化活性、总酚含量和总黄酮含量。Pearson 法分析 TPC 和 TFC 与抗氧化活性的相关性以及两种抗氧化测定方法之间的相关性。

结果

用 DPPH 法测定三种紫甘薯器官的抗氧化活性,其值范围为 6.572-290.894 mg AAE g1;用 CUPRAC 法测定,其值范围为 25.169-621.254 mg AAE g1。乙醇叶提取物的 TPC 最高(20.885 g GAE 100 g1),乙酸乙酯叶提取物的 TFC 最高(10.048 g QE 100 g1)。

结论

DPPH 和 CUPRAC 试验表明,紫甘薯叶、茎和块根提取物均具有较强的抗氧化能力。紫甘薯的废弃物(叶和茎)具有潜在的抗氧化活性。酚类和类黄酮化合物对其抗氧化活性有贡献。DPPH 和 CUPRAC 法对三种紫甘薯器官的大部分抗氧化活性均呈线性关系。乙醇茎提取物含有芦丁 7-O-葡萄糖苷、芦丁、槲皮素、山奈酚和芹菜素。芦丁含量最高,为 0.399%。

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