Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, 45320 Islamabad, Pakistan.
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, 45320 Islamabad, Pakistan.
Biomed Res Int. 2019 Apr 14;2019:8939854. doi: 10.1155/2019/8939854. eCollection 2019.
Sweet potato ( L. Lam.), known as "Shakarqandi" in Pakistan, is an imperative root vegetable with large size, traditionally used as aphrodisiac, antiprostatic, anti-inflammatory, antidiabetic, cardiotonic, and anticancer agent. Present study was conducted to gauge aphrodisiac potential of ethyl acetate (IPT-EA, IPA-EA) and methanol (IPT-M, IPA-M) extracts from tuber and aerial part, respectively, via behavioral and biochemical tests and their possible protective role in BPA-induced gonadotoxicity at the dose 300 mg/kg in male Sprague Dawley rats. Phytochemical analysis was done qualitatively and quantitatively through total phenolic and flavonoid content (TPC and TFC) and high performance liquid chromatographic (HPLC-DAD) fingerprinting while antioxidant profiling used multimode assays. To calculate sexual excitement mount latency, intromission latency, mount frequency, intromission frequency, ejaculatory latency, and postejaculatory interval were examined while for biochemical ratification semen characteristics, levels of testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol were measured. Gonadoprotective ability was assessed through comet assay and histomorphological examination of testes. Qualitative analysis ensured the presence of phenols, flavonoids, tannins, anthocyanin, saponins, coumarins, terpenoids, and betacyanin. Quantitatively maximal TPC (304.32±7.20 g GAE/mg dry extract) and TFC (214.77±4.09 g QE/mg DE) were estimated in IPA-EA extract. IPT-EA yielded maximum rutin (7.3±0.12) and myricetin (2.7±0.14 g/mg DE) while IPA-EA and IPA-M yielded maximum caffeic acid (4.05±0.22 and 1.92±0.17 g/mg DE, respectively) in HPLC-DAD analysis. Extracts enhanced sexual excitement, improved semen quality, levels of testosterone, FSH, LH, and estradiol, and successfully attenuated toxic effects of BPA. Levels of endogenous antioxidant enzymes (CAT, SOD, POD, and GSH) were restored and NO abundance was minimized. Significant stimulation in sexual behavior, amelioration of toxicity symptoms, elevated spermatic production, raised viability, vitalized levels of gonadal hormones, maintained endogenous enzymes, genoprotection, and reformed testicular histology endorsed as a better aphrodisiac alternative and gonadoprotective agent.
甘薯（L. Lam.），在巴基斯坦被称为“Shakarqandi”，是一种重要的根茎类蔬菜，具有大尺寸，传统上用作壮阳药、抗前列腺、抗炎、抗糖尿病、强心和抗癌剂。本研究旨在通过行为和生化测试评估来自块茎和地上部分的乙酸乙酯（IPT-EA、IPA-EA）和甲醇（IPT-M、IPA-M）提取物的壮阳潜力，并在 300mg/kg 剂量下评估其对 BPA 诱导的雄性 Sprague Dawley 大鼠生殖毒性的可能保护作用。通过总酚和类黄酮含量（TPC 和 TFC）和高效液相色谱-二极管阵列（HPLC-DAD）指纹图谱进行定性和定量的植物化学分析，同时使用多模式测定法进行抗氧化分析。为了计算性兴奋的勃起潜伏期、插入潜伏期、勃起频率、插入频率、射精潜伏期和射精后间隔，检查了精液特征、睾酮、卵泡刺激素（FSH）、黄体生成素（LH）和雌二醇水平。通过彗星试验和睾丸组织形态学检查评估性腺保护能力。定性分析确保存在酚类、类黄酮、单宁、花青素、皂苷、香豆素、萜类化合物和甜菜红素。定量分析结果表明，IPA-EA 提取物中的最大 TPC（304.32±7.20g GAE/mg 干提取物）和 TFC（214.77±4.09g QE/mg DE）最高。IPT-EA 产生的芦丁（7.3±0.12）和杨梅素（2.7±0.14g/mg DE）最高，而 IPA-EA 和 IPA-M 产生的咖啡酸（4.05±0.22 和 1.92±0.17g/mg DE）最高。提取物增强了性兴奋，改善了精液质量、睾酮、FSH、LH 和雌二醇水平，并成功减轻了 BPA 的毒性作用。内源性抗氧化酶（CAT、SOD、POD 和 GSH）水平得到恢复，NO 含量减少。性行为显著刺激、毒性症状改善、精子产生增加、活力提高、性腺激素水平升高、内源性酶维持、基因保护和睾丸组织学重建，被认为是一种更好的壮阳替代品和性腺保护剂。
