Impact Fed Larvae on Residues (Tramadol, Sildenafil Citrate and Diazepam) on Enzymes.

<b>Background and Objective:</b> <i>Chrysomya albiceps</i> are the insects most commonly associated with corpses, they are of medical and forensic value and thus their immature stages are used to estimate the post mortem interval (PMI) and may provide medicolegal experts essential information regardless of the time, place and cause of death. The current study's goal was to determine the impacts of these pharmaceuticals compounds in some of the biochemical parameters enzymes of the <i>C. albiceps</i> third instar larvae. <b>Materials and Methods:</b> The experiment was performed from April to June, 2018. <i>C. albiceps</i> was fed rabbit tissues during its development for three generations tramadol is administered an overdose of (30.8 mg kg¹), sildenafil citrate an overdose of (10.26 mg kg¹) and diazepam an overdose of dissolved in distilled water (4.06 mg kg¹), by intra-injection into the abdominal cavity twice daily for one week and rabbits injected with distilled water were also used to raise the control group. <i>Chrysomya albiceps</i> larvae in their third instars measured enzymes by using a spectrophotometer. <b>Results:</b> The results indicated to significantly increase of antioxidant enzymes activity of control group of TP, TAC, SOD, GST and CAT and decrease of MDA and significantly decreased of TP, TAC, SOD, GST and CAT and increase of MDA for the tramadol, sildenafil and diazepam groups for the three generations of larvae, respectively. <b>Conclusion:</b> In conclusion, the evidence suggested that decreased antioxidant enzyme activity and increase of MDA might be involved in the production of free radicals in <i>C. albiceps</i> oxidative stress is caused by these pharmaceuticals compounds.