Pak J Biol Sci. 2022 Jan;25(8):748-754. doi: 10.3923/pjbs.2022.748.754.
<b>Background and Objective:</b> <i>Colletotrichum</i> sp., is a pathogen that causes anthracnose disease that can reduce chilli production. One environmentally for controlling plant disease can be done using chitosan and silica nanotechnology. This study aimed to test the ability of chitosan and silica nanoparticles to inhibit the growth of <i>Colletotrichum</i> sp. and suppress the development of the diseases on chilli seeds. <b>Materials and Methods:</b> This research consisted of the pathogenicity test of chitosan and silica nanoparticles and chilli seed germination inhibition test to control the development of <i>Colletotrichum</i> sp., using a completely randomized design within 10 treatments and 3 replications. <b>Results:</b> The results showed that 100 ppm chitosan nanoparticles inhibited the growth of <i>Colletotrichum</i> sp. and conidia germination with inhibition percentages of 92.20 and 99.4%, respectively. In addition, the development of anthracnose on chilli seed germination has been suppressed by 93% at a 100 ppm concentration of silica nanoparticles. <b>Conclusion:</b> In conclusion, both single or mixed formulations of chitosan and silica nanoparticles were able to inhibit the growth and development of <i>Colletotrichum </i>sp. and increase the chilli seed viability.
炭疽菌是一种病原菌，可引起炭疽病，从而降低辣椒产量。一种环境友好的植物病害防治方法可以使用壳聚糖和纳米硅技术。本研究旨在测试壳聚糖和纳米硅颗粒抑制炭疽菌生长和抑制辣椒种子发病的能力。
本研究采用完全随机设计，设 10 个处理，3 次重复，包括壳聚糖和纳米硅颗粒的致病性试验和抑制辣椒种子发芽的试验，以控制炭疽菌的发展。
结果表明，100 ppm 壳聚糖纳米颗粒抑制了炭疽菌的生长和分生孢子的萌发，抑制率分别为 92.20%和 99.4%。此外，100 ppm 浓度的纳米硅颗粒抑制了炭疽病在辣椒种子发芽中的发展，抑制率达到 93%。
综上所述，壳聚糖和纳米硅的单一或混合配方均能抑制炭疽菌的生长和发育，提高辣椒种子活力。
