College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
Nat Prod Res. 2023 Jun;37(11):1902-1906. doi: 10.1080/14786419.2022.2123480. Epub 2022 Sep 13.
Presl (cinnamon), an important folk medicine is widely used to prevent osteoporosis for long time in China. Our study aimed to investigate the anti-osteoporosis activity and mechanisms of cinnamon extracts obtained by supercritical CO extraction (SFE) and identify activity associated chemical components by gas chromatography-mass spectrometry. The cinnamon SFE exhibited superior anti-osteoporosis efficacy in an ovariectomised mice model to common alcohol extracts. It could induce calcified nodules and ALP activity, upregulate the mRNA expression of ALP, BMP-2, and RUNX2 in MC3T3-E1 cells. The major chemical classes of cinnamon extracts were alcohol esters (28.2%), and terpenes (16.1%). The spectrum-activity analysis indicated that the potential chemical-markers of extracts could be (E)-Cinnamaldehyde, γ-Sitosterol, and (Z, Z)-9,12-Octadecadienoic acid, which could induce the proliferation and ALP activity in MC3T3-E1 cells. Our study revealed the promising applications of the cinnamon SFE in prevention of osteoporosis, and identified its anti-osteoporosis associated compounds.
桂皮（肉桂）作为一种重要的民间药物，在中国被广泛用于预防骨质疏松症已有很长时间。我们的研究旨在探讨超临界 CO 萃取（SFE）得到的桂皮提取物的抗骨质疏松活性和机制，并通过气相色谱-质谱联用技术鉴定与活性相关的化学成分。桂皮 SFE 在去卵巢小鼠模型中表现出比普通醇提物更优异的抗骨质疏松功效。它可以诱导钙化结节和 ALP 活性，上调 MC3T3-E1 细胞中 ALP、BMP-2 和 RUNX2 的 mRNA 表达。桂皮提取物的主要化学类分为醇酯（28.2%）和萜烯（16.1%）。谱效分析表明，提取物的潜在化学标志物可能是（E）-肉桂醛、γ-谷甾醇和（Z，Z）-9,12-十八碳二烯酸，它们可以诱导 MC3T3-E1 细胞的增殖和 ALP 活性。本研究揭示了桂皮 SFE 在预防骨质疏松症方面的应用前景，并鉴定了其与抗骨质疏松症相关的化合物。
