水提和醇提迷迭香对雄性小鼠生殖系统的影响：一项实验研究。

Effects of aqueous and hydro-alcoholic extracts of Seidlitzia rosmarinus on male mouse reproductive system: An experimental study.

机构信息

Department of Physiology, Faculty of Medicine, Student Research Committee, Dezful University of Medical Sciences, Dezful, Iran.

Department of Physiology, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran.

出版信息

JBRA Assist Reprod. 2023 Jun 22;27(2):254-258. doi: 10.5935/1518-0557.20220028.

DOI:10.5935/1518-0557.20220028

PMID:36098457

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10279448/

Abstract

OBJECTIVE

This study investigated the effects of aqueous and hydro-alcoholic extracts of Seidlitzia rosmarinus on reproductive hormones, sperm variables, and antioxidant enzymes level in the mice testis.

METHODS

In this experimental study, 24 three-month-old male NMRI mice weighing (25-30g) were divided into three groups: control, aqueous and hydro-alcoholic extracts of Seidlitzia rosmarinus 100mg/kg. Dissolved extracts were gavaged orally for 35 days. One day after receiving the last dose of the extract, the blood sample, testis, and the epididymis tail were taken for plasma hormonal, testicular antioxidants level, sperm count, and vitality assessments.

RESULTS

Testicular level of malondialdehyde increased in aqueous and hydro-alcoholic extracts groups (p=0.04); total antioxidant capacity decreased in aqueous and hydro-alcoholic extracts groups (p=0.008); and the consumption of aqueous (p<0.001) and hydro-alcoholic (p=0.03) extracts decreased catalase in comparison with the control group. The plasma level of luteinizing hormone decreased in the aqueous extracts administrated group (p=0.009); the follicle-stimulating hormone increased in aqueous (p=0.03), and hydro-alcoholic extracts administered mice; and the testosterone level decreased in aqueous extract-treated animals versus the control group (p<0.001). The sperm count was increased in aqueous (p=0.04) and hydro-alcoholic (p=0.009) extracts groups, but its vitality was decreased (p=0.008) in comparison with the control group.

CONCLUSIONS

In conclusion, Seidlitzia rosmarinus has an adverse effect on male reproductive hormones and sperm viability via increased lipid peroxidation and reduced antioxidant defense system performance.

摘要

目的

本研究旨在探讨 Seidlitzia rosmarinus 的水提物和水醇提物对小鼠睾丸生殖激素、精子变量和抗氧化酶水平的影响。

方法

在这项实验研究中，将 24 只 3 月龄、体重（25-30g）为 NMRI 雄性小鼠分为三组：对照组、Seidlitzia rosmarinus 水提物 100mg/kg 组和 Seidlitzia rosmarinus 水醇提物 100mg/kg 组。将溶解的提取物灌胃 35 天。末次给药 1 天后，采集血样、睾丸和附睾尾，用于评估血浆激素、睾丸抗氧化水平、精子计数和活力。

结果

水提物和水醇提物组睾丸丙二醛水平升高（p=0.04）；水提物和水醇提物组总抗氧化能力降低（p=0.008）；与对照组相比，水提物（p<0.001）和水醇提物（p=0.03）组的过氧化氢酶消耗减少。水提物组黄体生成素（LH）血浆水平降低（p=0.009）；水提物和水醇提物组促卵泡激素（FSH）增加（p=0.03）；水提物组睾丸酮（T）水平降低（p<0.001）。与对照组相比，水提物（p=0.04）和水醇提物（p=0.009）组的精子计数增加，但活力降低（p=0.008）。