Leiden University Medical Center, Center for Proteomics and Metabolomics, 2300 RC Leiden, The Netherlands.
Anal Chem. 2022 Sep 27;94(38):12954-12959. doi: 10.1021/acs.analchem.2c02840. Epub 2022 Sep 13.
Isomeric -glycans often vastly differ in their biological activities, hence the need for methods that allow resolving and structurally characterizing them in biological material. Here, we established a zero flow approach using capillary electrophoresis in combination with (tandem) mass spectrometry to allow structural characterization of isomeric -glycans at high sensitivity. Additionally, diagnostic fragment ion ratios were identified, indicative for the antenna carrying specifically linked sialic acids. In total, 208 -glycans were characterized in human plasma, with 57 compositions showing multiple isomers.
在生物体内，糖蛋白中糖链的同分异构体在生物学活性上可能存在巨大差异，因此需要开发能够对其进行分离和结构鉴定的方法。本研究建立了一种基于毛细管电泳与（串联）质谱联用的零流进样方法，可实现对生物样本中同分异构体糖链的高灵敏度结构鉴定。此外，还鉴定出了具有特定连接唾液酸的天线的特征性诊断碎片离子比。本研究共鉴定了 208 种人血浆中的糖蛋白聚糖，其中 57 种具有多个同分异构体。
