CD3CD56 T 淋巴细胞与 1 型糖尿病中的内质网应激和炎性小体激活有关。
CD3CD56 T Lymphocytes Are Associated With ER Stress and Inflammasome Activation in Type 1 Diabetes.
机构信息
Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Division of Genetics and Metabolism, Children's Hospital of China Medical University, Taichung, Taiwan, R.O.C.
出版信息
In Vivo. 2022 Sep-Oct;36(5):2083-2091. doi: 10.21873/invivo.12934.
BACKGROUND/AIM: The T cell's flexibility of the immune system to be regulated affects the onset of type 1 diabetes (T1D). However, the mechanisms of endoplasmic reticulum (ER) stress and inflammasome activation in the circulating CD3CD56 T cells of patients with T1D remain unclear. This study evaluated the role of CD3CD56 T cells in T1D and their correlations with ER stress, inflammasome activation and disease characteristics.
MATERIALS AND METHODS
The frequency of circulating CD3CD56 T cells was determined using flow cytometry in healthy individuals and patients with T1D. Calnexin, NLR family pyrin domain containing 3 (NLRP3), ASC, caspase-1 (Casp1), cleaved caspase-3 (C-Casp3), and annexin V (AnnV) expression and propidium iodide staining in CD3/CD56 T cells were analyzed using flow cytometry.
RESULTS
The frequency of CD3CD56 T cells was reduced in patients with T1D relative to that in healthy individuals. In addition, high calnexin, NLRP3, ASC and Casp1 expression in CD3CD56 T cells was negatively correlated with the percentage of CD3CD56 T cells in patients with T1D.
CONCLUSION
ER stress, inflammasome activation, and a lower peripheral frequency of circulating CD3CD56 T cells might indicate disease progression and necessitate clinical T1D immunological self-tolerance monitoring.
背景/目的：免疫系统中 T 细胞的灵活性受到调节，这会影响 1 型糖尿病（T1D）的发病。然而，T1D 患者循环 CD3CD56 T 细胞中内质网（ER）应激和炎性体激活的机制尚不清楚。本研究评估了 CD3CD56 T 细胞在 T1D 中的作用及其与 ER 应激、炎性体激活和疾病特征的相关性。
材料和方法
采用流式细胞术检测健康个体和 T1D 患者循环 CD3CD56 T 细胞的频率。采用流式细胞术分析 CD3/CD56 T 细胞中钙网织蛋白、NLR 家族 pyrin 结构域包含 3（NLRP3）、ASC、半胱天冬酶-1（Casp1）、裂解的半胱天冬酶-3（C-Casp3）和膜联蛋白 V（AnnV）的表达和碘化丙啶染色。
结果
与健康个体相比，T1D 患者 CD3CD56 T 细胞的频率降低。此外，CD3CD56 T 细胞中高钙网织蛋白、NLRP3、ASC 和 Casp1 表达与 T1D 患者 CD3CD56 T 细胞的百分比呈负相关。
结论
ER 应激、炎性体激活以及外周循环 CD3CD56 T 细胞频率降低可能提示疾病进展，需要对 T1D 进行临床免疫自身耐受监测。
Zotero 插件