Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.
Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan;
In Vivo. 2022 Sep-Oct;36(5):2211-2217. doi: 10.21873/invivo.12947.
BACKGROUND/AIM: Acid-electrolyzed functional water (FW) is an efficient bactericide and gargling with FW might be an effective method of oral care. We investigated the possible use of FW as a mouth wash by an in vitro study.
The bactericidal effect of FW against different species of bacteria (Staphylococcus aureus, Streptococcus pneumonia, Pseudomonas aeruginosa, and Candida albicans) was evaluated using the numbers of colony-forming units (CFU). The experiment was conducted using PBS, LISTERINE, and ConCool F (undiluted, and the optimal concentration indicated). To investigate the bactericidal mechanism of FW, the activity of superoxide dismutase (SOD), an indicator of oxidative action, was measured in S. aureus. FW was diluted with purified water to concentrations of 10, 30, 50, and 70%. The numbers of CFU were measured for each concentration. XTT assays were performed using HSC-3 and HeLa cells, to examine the viability of the cells following treatment with FW. The same experiment was conducted with PBS, LISTERINE, and undiluted ConCool F.
No bacteria treated with FW formed colonies. SOD activity peaked at a 50% concentration of FW and was more than twice that of the control. A significant decrease in the number of CFU was observed following 50% treatment. Since the peaks of the SOD activity and the starting concentrations of the bactericidal effects coincided, the bactericidal effect of FW might be related to its oxidative effects. Bacteria treated with FW had the same survival rate as the other mouth washes.
FW might be clinically applicable as a mouth wash.
背景/目的：酸电解功能水（FW）是一种高效的杀菌剂，漱口 FW 可能是一种有效的口腔护理方法。我们通过体外研究来探讨 FW 作为漱口液的可能性。
使用菌落形成单位（CFU）评估 FW 对不同种类细菌（金黄色葡萄球菌、肺炎链球菌、铜绿假单胞菌和白色念珠菌）的杀菌效果。实验使用 PBS、LISTERINE 和 ConCool F（未稀释和最佳浓度）进行。为了研究 FW 的杀菌机制，测量了金黄色葡萄球菌中超氧化物歧化酶（SOD）的活性，SOD 是氧化作用的指标。将 FW 用纯净水稀释至 10、30、50 和 70%。测量每个浓度的 CFU 数。使用 HSC-3 和 HeLa 细胞进行 XTT 测定，以检查 FW 处理后细胞的活力。用 PBS、LISTERINE 和未稀释的 ConCool F 进行相同的实验。
没有用 FW 处理的细菌形成菌落。SOD 活性在 FW 的 50%浓度下达到峰值，是对照的两倍多。用 50%FW 处理后 CFU 数量明显减少。由于 SOD 活性峰值和杀菌效果的起始浓度相吻合，FW 的杀菌效果可能与其氧化作用有关。用 FW 处理的细菌与其他漱口液的存活率相同。
FW 可能在临床上适用于作为漱口液。
