酸化电解功能水用于漱口的效果：一项研究。

Effectiveness of Acid-electrolyzed Functional Water for Mouth Wash: An Study.

机构信息

Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.

Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan;

出版信息

In Vivo. 2022 Sep-Oct;36(5):2211-2217. doi: 10.21873/invivo.12947.

DOI:10.21873/invivo.12947

PMID:36099114

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9463900/

Abstract

BACKGROUND/AIM: Acid-electrolyzed functional water (FW) is an efficient bactericide and gargling with FW might be an effective method of oral care. We investigated the possible use of FW as a mouth wash by an in vitro study.

MATERIALS AND METHODS

The bactericidal effect of FW against different species of bacteria (Staphylococcus aureus, Streptococcus pneumonia, Pseudomonas aeruginosa, and Candida albicans) was evaluated using the numbers of colony-forming units (CFU). The experiment was conducted using PBS, LISTERINE, and ConCool F (undiluted, and the optimal concentration indicated). To investigate the bactericidal mechanism of FW, the activity of superoxide dismutase (SOD), an indicator of oxidative action, was measured in S. aureus. FW was diluted with purified water to concentrations of 10, 30, 50, and 70%. The numbers of CFU were measured for each concentration. XTT assays were performed using HSC-3 and HeLa cells, to examine the viability of the cells following treatment with FW. The same experiment was conducted with PBS, LISTERINE, and undiluted ConCool F.

RESULTS

No bacteria treated with FW formed colonies. SOD activity peaked at a 50% concentration of FW and was more than twice that of the control. A significant decrease in the number of CFU was observed following 50% treatment. Since the peaks of the SOD activity and the starting concentrations of the bactericidal effects coincided, the bactericidal effect of FW might be related to its oxidative effects. Bacteria treated with FW had the same survival rate as the other mouth washes.

CONCLUSION

FW might be clinically applicable as a mouth wash.

摘要

背景/目的：酸电解功能水（FW）是一种高效的杀菌剂，漱口 FW 可能是一种有效的口腔护理方法。我们通过体外研究来探讨 FW 作为漱口液的可能性。

材料和方法

使用菌落形成单位（CFU）评估 FW 对不同种类细菌（金黄色葡萄球菌、肺炎链球菌、铜绿假单胞菌和白色念珠菌）的杀菌效果。实验使用 PBS、LISTERINE 和 ConCool F（未稀释和最佳浓度）进行。为了研究 FW 的杀菌机制，测量了金黄色葡萄球菌中超氧化物歧化酶（SOD）的活性，SOD 是氧化作用的指标。将 FW 用纯净水稀释至 10、30、50 和 70%。测量每个浓度的 CFU 数。使用 HSC-3 和 HeLa 细胞进行 XTT 测定，以检查 FW 处理后细胞的活力。用 PBS、LISTERINE 和未稀释的 ConCool F 进行相同的实验。