Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
National Engineering Research Center for Beijing Biochip Technology, Beijing, 102206, China.
ACS Nano. 2022 Sep 27;16(9):14849-14859. doi: 10.1021/acsnano.2c05752. Epub 2022 Sep 13.
Nitrite plays a critical role in a variety of physiological processes and maintaining the nitrite level in an appropriate range is vital to keep healthy. Current nitrite analysis methods lack sensitivity and require tedious operations, which could not meet the need of point-of-care (POC) nitrite detection in precision medicine. Here we present a cyanocobalamin (VB) bionic enzyme-assisted ion-selective amperometric biosensor based on 3D porous conductive matrix (PCM), which can facilitate rapid and accurate POC nitrite monitoring in complex biofluids. The experimental findings quantitatively demonstrate that the biosensor has a sensitivity of 64.08 μA/(mM·cm), a wide linear range of 0.025-45 mM, and low limit of detection of 1 nM. Moreover, the developed VB/BSA-PCM biosensor shows outstanding stability after 21 days with 2% decline in current signal, and high repeatability between batches with RSD of only 1.29%. Real salivary nitrite detection has been evaluated, and the results match well with the commercial nitrite analyzer. Thus, the bionic enzyme-assisted ion-selective amperometric biosensor proposed herein has potential utility as an affordable tool for POC detection and home-based healthcare.
亚硝酸盐在各种生理过程中起着关键作用，将亚硝酸盐水平维持在适当范围内对保持健康至关重要。目前的亚硝酸盐分析方法缺乏灵敏度，需要繁琐的操作，无法满足精准医学中即时检测（POC）亚硝酸盐的需求。在这里，我们提出了一种基于 3D 多孔导电基质（PCM）的氰钴胺素（VB）仿生酶辅助离子选择性安培生物传感器，可促进在复杂生物流体中快速准确地进行即时检测。实验结果定量证明，该生物传感器具有 64.08 μA/（mM·cm）的灵敏度、0.025-45 mM 的宽线性范围和低至 1 nM 的检测限。此外，开发的 VB/BSA-PCM 生物传感器在电流信号下降 2%的情况下经过 21 天后仍表现出出色的稳定性，批次间具有很高的重复性，RSD 仅为 1.29%。已经对真实唾液中亚硝酸盐的检测进行了评估，结果与商业亚硝酸盐分析仪吻合良好。因此，本文提出的仿生酶辅助离子选择性安培生物传感器具有作为一种经济实惠的即时检测和家庭医疗工具的潜力。
