Department of Materials Science and Engineering, University of Texas at Dallas, Richardson, TX 75080, USA; Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 100-715, Republic of Korea.
Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Incheon 402-751, Republic of Korea.
Biosens Bioelectron. 2016 May 15;79:543-52. doi: 10.1016/j.bios.2015.12.054. Epub 2015 Dec 19.
In this report, titanium nitride (TiN) nanoparticles decorated multi-walled carbon nanotube (MWCNTs) nanocomposite is fabricated via a two-step process. These two steps involve the decoration of titanium dioxide nanoparticles onto the MWCNTs surface and a subsequent thermal nitridation. Transmission electron microscopy shows that TiN nanoparticles with a mean diameter of ≤ 20 nm are homogeneously dispersed onto the MWCNTs surface. Direct electrochemistry and electrocatalysis of cytochrome c immobilized on the MWCNTs-TiN composite modified on a glassy carbon electrode for nitrite sensing are investigated. Under optimum conditions, the current response is linear to its concentration from 1 µM to 2000 µM with a sensitivity of 121.5 µA µM(-1)cm(-2) and a low detection limit of 0.0014 µM. The proposed electrode shows good reproducibility and long-term stability. The applicability of the as-prepared biosensor is validated by the successful detection of nitrite in tap and sea water samples.
在本报告中，通过两步法制备了氮化钛（TiN）纳米粒子修饰的多壁碳纳米管（MWCNTs）纳米复合材料。这两步包括将二氧化钛纳米粒子修饰到 MWCNTs 表面，以及随后的热氮化。透射电子显微镜显示，平均直径≤20nm 的 TiN 纳米粒子均匀分散在 MWCNTs 表面。通过直接电化学和固定在 MWCNTs-TiN 复合修饰玻碳电极上的细胞色素 c 的电催化研究了亚硝酸盐的传感。在最佳条件下，电流响应在 1µM 至 2000µM 范围内呈线性，灵敏度为 121.5µAµM(-1)cm(-2)，检测限低至 0.0014µM。所制备的电极具有良好的重现性和长期稳定性。通过成功检测自来水中和海水中的亚硝酸盐验证了所制备的生物传感器的适用性。
