Federal State Educational Institution of Higher Education «Rostov State Medical University» Ministry of Health of Russia.
Rostov-on-Don Plague Control Research Institute.
Klin Lab Diagn. 2022 Sep 12;67(9):519-524. doi: 10.51620/0869-2084-2022-67-9-519-524.
Corynebacterium spp. are part of the human microbiome, but can cause the development of inflammatory diseases of various localization. Purpose - to evaluate the relationship between pathogenic properties and resistance to antimicrobial drugs (AMD) of Corynebacterium spp. from patients with inflammatory diseases of the respiratory tract. Strains of Corynebacterium spp. isolated from patients with inflammatory diseases of the respiratory tract (99 pcs.) and practically healthy individuals (33 pcs.). Isolates were identified by mass spectrometric method (MALDI-ToFMS), their adhesive and invasive activity on Hep-2 cells, cytopathic effect (CPE) in CHO-K1 cell culture, and resistance to antimicrobial drugs (AMD) were determined. Indicators of adhesion (3.65±0.679(CFU±m)x102/ml), invasion (1.72±0.230 (CFU±m)x102/ml), cytotoxicity (69.1±3.8% of dead CHO-K1 cells ) Corynebasterium spp. strains isolated from patients are higher (p≤0.05) than similar indicators in practically healthy people. 90.9% of isolates from patients had resistance to AMD, in most cases (57.6±4.9%) resistance to only one AMP was noted, less often to two (25.2±4.3%), three or more (8.08±2.7%). According to the results of correlation-regression analysis, pathogenic properties (adhesiveness, invasiveness, cytotoxicity) of Corynebacterium spp. strains isolated from patients are in close direct relationship with resistance to AMD. This indicates the importance of identifying strains of non-diphtheria corynebacteria resistant to AMDs, which, under the influence of developing resistance to AMDs, can increase their pathogenic potential, moving from commensalism to parasitism.
棒状杆菌属是人类微生物组的一部分，但可引起各种定位的炎症性疾病的发展。目的-评估呼吸道炎症性疾病患者的棒状杆菌属的致病性和对抗菌药物（AMD）的耐药性之间的关系。从呼吸道炎症性疾病患者（99 例）和健康个体（33 例）中分离出棒状杆菌属菌株。通过质谱法（MALDI-ToFMS）鉴定分离株，测定其在 Hep-2 细胞上的粘附和侵袭活性，CHO-K1 细胞培养中的细胞病变效应（CPE）以及对抗菌药物（AMD）的耐药性。粘附（3.65±0.679（CFU±m）x102/ml），侵袭（1.72±0.230（CFU±m）x102/ml）和细胞毒性（69.1±3.8％死亡的 CHO-K1 细胞）指标 Corynebacterium spp. 分离自患者的菌株高于（p≤0.05）健康人。 90.9％的患者分离株对 AMD 具有耐药性，在大多数情况下（57.6±4.9％）仅对一种 AMP 有耐药性，较少对两种（25.2±4.3％），三种或更多（8.08±2.7％）。根据相关回归分析的结果，分离自患者的 Corynebacterium spp.菌株的致病性（粘附性，侵袭性，细胞毒性）与 AMD 耐药性密切相关。这表明确定对 AMD 具有耐药性的非白喉棒状杆菌菌株的重要性，在对 AMD 产生耐药性的影响下，这些菌株可以从共生状态转变为寄生状态，从而增加其致病潜力。
