Kusumoto K
Hokkaido Igaku Zasshi. 1987 Mar;62(2):220-9.
If the isolated liver could be preserved at subzero temperatures, it would keep viability for a longer time. But the liver is so large that it is difficult to cool and rewarm with an uniform heat distribution for minimizing differential effects of biochemical reactions. A new system was developed in order to cool and rewarm the liver uniformly. The apparatus consists of two heat exchangers, one temperature controller using a computer system and one hypothermic bath. The temperature of a perfusate and an immersing solution was controlled at the same time. Wistar rat livers were cooled to -4 degrees C and rewarmed to +4 degrees C by this system using 10% DMSO (Me2SO) solution. The perfusion pressure was 8-10 cmH2O and the liver temperature was uniformly controlled when the perfusion flow rate was 0.1 ml/g X liver/min and the temperature gradient was programmed at 2 degrees C/min. Levels of adenine nucleotides and histologic findings were evaluated in the livers which were cooled to -4 degrees C by this system and preserved for 24 hours. Levels of adenine nucleotides were higher in the liver preserved with -4 degrees C 10% DMSO Collins' M solution for 24 hours compared with those of +4 degrees C Collins' M for 24 hours and +4 degrees C lactated Ringer solution for 1 or 2 hours. Light microscopic findings of the liver preserved at -4 degrees C for 24 hours showed slight cellular atrophy and sinusoidal dilatation. But cellular and vascular structures appeared to be almost normally maintained. The liver preserved at +4 degrees C for 24 hours showed diffuse necrosis of liver cells and architectural destruction of the lobules. This system was useful for controlling the liver temperature in cooling and rewarming. The viability of livers at subzero temperatures controlled with this system was better than those above 0 degree C. This method may prolong the preservation period of the liver with a good viability.
如果离体肝脏能够在零下温度下保存，其存活时间将会更长。但肝脏体积巨大，难以实现均匀降温与复温，以尽量减少生化反应的差异效应。为实现肝脏的均匀降温和复温，研发了一种新系统。该装置由两个热交换器、一个使用计算机系统的温度控制器和一个低温浴槽组成。同时控制灌注液和浸泡液的温度。使用10%二甲基亚砜（Me2SO）溶液，通过该系统将Wistar大鼠肝脏冷却至-4℃，然后复温至+4℃。当灌注流速为0.1 ml/g×肝脏/分钟且温度梯度设定为2℃/分钟时，灌注压力为8-10 cmH2O，肝脏温度得到均匀控制。对通过该系统冷却至-4℃并保存24小时的肝脏进行腺嘌呤核苷酸水平和组织学检查。与在+4℃柯林斯M溶液中保存24小时以及在+4℃乳酸林格液中保存1或2小时的肝脏相比，在-4℃ 10%二甲基亚砜柯林斯M溶液中保存24小时的肝脏中腺嘌呤核苷酸水平更高。在-4℃保存24小时的肝脏的光镜检查结果显示有轻微的细胞萎缩和窦状隙扩张。但细胞和血管结构似乎几乎保持正常。在+4℃保存24小时的肝脏显示肝细胞弥漫性坏死和肝小叶结构破坏。该系统有助于在降温和复温过程中控制肝脏温度。用该系统控制的零下温度下肝脏的存活能力优于0℃以上的肝脏。这种方法可能会延长肝脏的保存期并保持良好的存活能力。
