Li Xuejuan, Lin Feng, Li Chenyang, Du Linlin, Liu Zhiyang, Shi Wenjuan, Lv Jianying, Cao Xiaoyan, Lan Ying, Fan Yongjian, Zhou Yijun, Zhou Tong
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu Province, China.
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China.
Theor Appl Genet. 2022 Dec;135(12):4233-4243. doi: 10.1007/s00122-022-04214-9. Epub 2022 Sep 13.
A major resistance QTL was identified on chromosome 6 in rice variety Wuke; both overexpression and knockdown experiments confirmed that OsGLK1 is the candidate gene for association with Rice black-streaked dwarf virus disease. Rice black-streaked dwarf virus disease is one of the most destructive rice viral diseases in China and East Asia. Progress has been limited in RBSDVD resistance breeding due to inadequate knowledge on the underlying functional genes. In this study, a major QTL for RBSDV (rice black-streaked dwarf virus) independent of SBPH (small brown planthopper) resistance was mapped in a 1.8 Mb interval on chromosome 6 by using an F population originated from resistant rice variety Wuke. Representative transcripts within this region were analysed and three genes showing amino acid sequence variation in functional domains were selected for transformation. Overexpression experiments showed that one gene exhibited significant enhanced resistance compared to control lines, encoding protein involving Myb domain and probable transcription factor Golden 2-like1 (GLK1). Furthermore, OsGLK1 knockdown rice lines were investigated and the resistance ability was significantly declined without this gene compared to the wild type. Taken together, both overexpression and knockdown experiments strongly suggested that OsGLK1 plays an important role for RBSDV resistance and contributes to the major QTL. The study paves the way for elucidating the molecular mechanism underlying RBSDVD resistance and the molecular markers associated with OsGLK1 may be used for marker-assisted selection.
在水稻品种武科的6号染色体上鉴定出一个主要的抗性QTL；过表达和基因敲低实验均证实，OsGLK1是与水稻黑条矮缩病毒病相关的候选基因。水稻黑条矮缩病毒病是中国和东亚最具破坏性的水稻病毒病之一。由于对潜在功能基因的了解不足，水稻黑条矮缩病毒病抗性育种进展有限。在本研究中，利用源自抗性水稻品种武科的F群体，在6号染色体上一个1.8 Mb的区间内定位了一个独立于灰飞虱抗性的水稻黑条矮缩病毒主要QTL。对该区域内的代表性转录本进行了分析，并选择了三个在功能域中显示氨基酸序列变异的基因进行转化。过表达实验表明，与对照品系相比，一个基因表现出显著增强的抗性，该基因编码的蛋白含有Myb结构域和可能的转录因子Golden 2-like1（GLK1）。此外，对OsGLK1基因敲低的水稻品系进行了研究，与野生型相比，没有该基因时抗性能力显著下降。综上所述，过表达和基因敲低实验均强烈表明，OsGLK1在水稻黑条矮缩病毒抗性中起重要作用，并对主要QTL有贡献。该研究为阐明水稻黑条矮缩病毒病抗性的分子机制铺平了道路，与OsGLK1相关的分子标记可用于标记辅助选择。
